Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 5028860..5029554 Replicon chromosome
Accession NZ_CP117959
Organism Escherichia coli strain B-2207

Toxin (Protein)


Gene name yafO Uniprot ID A0A3P5GWS5
Locus tag PT284_RS25055 Protein ID WP_001263488.1
Coordinates 5029156..5029554 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag PT284_RS25050 Protein ID WP_000554758.1
Coordinates 5028860..5029153 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PT284_RS25030 (5024491) 5024491..5024989 + 499 Protein_4738 REP-associated tyrosine transposase RayT -
PT284_RS25035 (5025213) 5025213..5026925 - 1713 Protein_4739 flagellar biosynthesis protein FlhA -
PT284_RS25040 (5026897) 5026897..5027682 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
PT284_RS25045 (5027753) 5027753..5028808 + 1056 WP_001226164.1 DNA polymerase IV -
PT284_RS25050 (5028860) 5028860..5029153 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
PT284_RS25055 (5029156) 5029156..5029554 + 399 WP_001263488.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
PT284_RS25060 (5029564) 5029564..5030016 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
PT284_RS25065 (5030334) 5030334..5030540 + 207 Protein_4745 RtcB family protein -
PT284_RS25070 (5030536) 5030536..5031057 + 522 Protein_4746 peptide chain release factor H -
PT284_RS25075 (5031114) 5031114..5032571 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
PT284_RS25080 (5032832) 5032832..5033290 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (5033886) 5033886..5033966 + 81 NuclAT_12 - -
- (5033886) 5033886..5033966 + 81 NuclAT_12 - -
- (5033886) 5033886..5033966 + 81 NuclAT_12 - -
- (5033886) 5033886..5033966 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15559.90 Da        Isoelectric Point: 6.9798

>T272513 WP_001263488.1 NZ_CP117959:5029156-5029554 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQEAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT272513 WP_000554758.1 NZ_CP117959:5028860-5029153 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3P5GWS5

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References