Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 5022815..5023356 Replicon chromosome
Accession NZ_CP117959
Organism Escherichia coli strain B-2207

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag PT284_RS25015 Protein ID WP_000615983.1
Coordinates 5022815..5023093 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag PT284_RS25020 Protein ID WP_000729703.1
Coordinates 5023096..5023356 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PT284_RS25000 (5020397) 5020397..5020975 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
PT284_RS25005 (5021181) 5021181..5021948 + 768 WP_000333380.1 class II glutamine amidotransferase -
PT284_RS25010 (5021919) 5021919..5022659 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
PT284_RS25015 (5022815) 5022815..5023093 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
PT284_RS25020 (5023096) 5023096..5023356 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
PT284_RS25025 (5023566) 5023566..5024315 + 750 WP_000056849.1 C40 family peptidase -
PT284_RS25030 (5024491) 5024491..5024989 + 499 Protein_4738 REP-associated tyrosine transposase RayT -
PT284_RS25035 (5025213) 5025213..5026925 - 1713 Protein_4739 flagellar biosynthesis protein FlhA -
PT284_RS25040 (5026897) 5026897..5027682 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T272512 WP_000615983.1 NZ_CP117959:c5023093-5022815 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT272512 WP_000729703.1 NZ_CP117959:c5023356-5023096 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References