Detailed information of TA system TA027250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4471198..4471800
• Replicon: chromosome
• Accession: NC_017628
• Organism: Escherichia coli IHE3034

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: ECOK1_RS22495
• Protein ID: WP_000897302.1
• Coordinates: 4471198..4471509 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ECOK1_RS22500
• Protein ID: WP_000356397.1
• Coordinates: 4471510..4471800 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECOK1_RS22470	4467112..4467711	+	600	WP_001314324.1	glucose-1-phosphatase	-
ECOK1_RS22475	4467705..4468577	+	873	WP_000920762.1	virulence factor BrkB family protein	-
ECOK1_RS22480	4468574..4469011	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
ECOK1_RS22485	4469056..4469997	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
ECOK1_RS22490	4470061..4470969	-	909	WP_001331553.1	alpha/beta hydrolase	-
ECOK1_RS22495	4471198..4471509	+	312	WP_000897302.1	hypothetical protein	Toxin
ECOK1_RS22500	4471510..4471800	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
ECOK1_RS22510	4472159..4472437	+	279	WP_001296612.1	hypothetical protein	-
ECOK1_RS22515	4472833..4473051	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
ECOK1_RS22520	4473267..4474196	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
ECOK1_RS22525	4474193..4474828	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
ECOK1_RS22530	4474825..4475727	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T27250 WP_000897302.1 NC_017628:4471198-4471509 [Escherichia coli IHE3034]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T27250 NC_017628:4471198-4471509 [Escherichia coli IHE3034]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT27250 WP_000356397.1 NC_017628:4471510-4471800 [Escherichia coli IHE3034]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT27250 NC_017628:4471510-4471800 [Escherichia coli IHE3034]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416