Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2305790..2306292 Replicon chromosome
Accession NC_017628
Organism Escherichia coli IHE3034

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag ECOK1_RS11770 Protein ID WP_000767822.1
Coordinates 2305790..2306044 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag ECOK1_RS11775 Protein ID WP_001259255.1
Coordinates 2306041..2306292 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECOK1_RS11755 2302283..2303641 - 1359 WP_000019194.1 putrescine/proton symporter PlaP -
ECOK1_RS28425 2303631..2303693 - 63 WP_010723108.1 membrane protein YoeI -
ECOK1_RS11760 2303908..2304837 - 930 WP_000803366.1 LysR family transcriptional regulator -
ECOK1_RS11765 2304883..2305707 - 825 WP_000754733.1 SDR family oxidoreductase -
ECOK1_RS11770 2305790..2306044 - 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
ECOK1_RS11775 2306041..2306292 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
ECOK1_RS26730 2306575..2306625 + 51 WP_001364200.1 his operon leader peptide -
ECOK1_RS11780 2306771..2307670 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
ECOK1_RS11785 2307676..2308980 + 1305 WP_001297912.1 histidinol dehydrogenase -
ECOK1_RS11790 2308977..2310047 + 1071 WP_000108995.1 histidinol-phosphate transaminase -
ECOK1_RS11795 2310047..2311114 + 1068 WP_000080122.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T27247 WP_000767822.1 NC_017628:c2306044-2305790 [Escherichia coli IHE3034]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T27247 NC_017628:c2306044-2305790 [Escherichia coli IHE3034]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT27247 WP_001259255.1 NC_017628:c2306292-2306041 [Escherichia coli IHE3034]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT27247 NC_017628:c2306292-2306041 [Escherichia coli IHE3034]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References