Detailed information of TA system TA027242

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hokW-rdlD/-
• Location: 2135624..2135849
• Replicon: chromosome
• Accession: NC_017628
• Organism: Escherichia coli IHE3034

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: ECOK1_RS11080
• Protein ID: WP_000813254.1
• Coordinates: 2135624..2135779 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	rdlD
Locus tag	-
Coordinates	2135791..2135849 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECOK1_RS11025	2130704..2131018	-	315	WP_000193273.1	YdfR family protein	-
ECOK1_RS11030	2131023..2131238	-	216	WP_000839572.1	class II holin family protein	-
ECOK1_RS11055	2131963..2132676	-	714	WP_000342820.1	hypothetical protein	-
ECOK1_RS11060	2132922..2133743	-	822	WP_001235237.1	antitermination protein	-
ECOK1_RS11065	2133740..2134114	-	375	WP_001217427.1	RusA family crossover junction endodeoxyribonuclease	-
ECOK1_RS11070	2134127..2135176	-	1050	WP_001265292.1	DUF968 domain-containing protein	-
ECOK1_RS27865	2135178..2135456	-	279	WP_024193993.1	hypothetical protein	-
ECOK1_RS11080	2135624..2135779	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	2135791..2135849	+	59	-	-	Antitoxin
ECOK1_RS11085	2136017..2136682	-	666	WP_000150294.1	epoxyqueuosine reductase QueH	-
ECOK1_RS11090	2136857..2137282	-	426	WP_001151152.1	DUF977 family protein	-
ECOK1_RS11095	2137323..2138393	-	1071	WP_001262355.1	hypothetical protein	-
ECOK1_RS11100	2138465..2138890	-	426	WP_000693918.1	Rha family transcriptional regulator	-
ECOK1_RS11105	2138874..2139146	-	273	WP_000887447.1	helix-turn-helix domain-containing protein	-
ECOK1_RS11110	2139256..2139657	+	402	WP_001329851.1	helix-turn-helix domain-containing protein	-
ECOK1_RS11115	2139685..2139876	+	192	WP_000100899.1	hypothetical protein	-
ECOK1_RS11120	2139876..2140163	+	288	WP_000936799.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ECOK1_RS11125	2140435..2140587	+	153	WP_000379609.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ybtS / ybtX / ybtQ / ybtP	2106750..2155801	49051

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T27242 WP_000813254.1 NC_017628:c2135779-2135624 [Escherichia coli IHE3034]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T27242 NC_017628:c2135779-2135624 [Escherichia coli IHE3034]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT27242 NC_017628:2135791-2135849 [Escherichia coli IHE3034]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin