Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1663231..1663793 Replicon chromosome
Accession NC_017628
Organism Escherichia coli IHE3034

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag ECOK1_RS08535 Protein ID WP_000605675.1
Coordinates 1663515..1663793 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag ECOK1_RS08530 Protein ID WP_000781370.1
Coordinates 1663231..1663515 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECOK1_RS08515 1658412..1661459 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
ECOK1_RS08520 1661472..1662356 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
ECOK1_RS08525 1662349..1663002 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
ECOK1_RS08530 1663231..1663515 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
ECOK1_RS08535 1663515..1663793 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ECOK1_RS08540 1663979..1664989 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
ECOK1_RS08545 1665123..1666820 - 1698 WP_000433462.1 malate dehydrogenase -
ECOK1_RS08550 1666976..1667113 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
ECOK1_RS08555 1667215..1667430 - 216 WP_000495766.1 biofilm-dependent modulation protein -
ECOK1_RS08560 1667775..1668206 + 432 WP_000152310.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T27232 WP_000605675.1 NC_017628:c1663793-1663515 [Escherichia coli IHE3034]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T27232 NC_017628:c1663793-1663515 [Escherichia coli IHE3034]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT27232 WP_000781370.1 NC_017628:c1663515-1663231 [Escherichia coli IHE3034]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT27232 NC_017628:c1663515-1663231 [Escherichia coli IHE3034]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCGGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References