Detailed information of TA system TA027229

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hokW-rdlD/-
• Location: 1444271..1444496
• Replicon: chromosome
• Accession: NC_017628
• Organism: Escherichia coli IHE3034

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A8S7B7V6
• Locus tag: ECOK1_RS07405
• Protein ID: WP_000813256.1
• Coordinates: 1444341..1444496 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	rdlD
Locus tag	-
Coordinates	1444271..1444329 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECOK1_RS07370	1439296..1439538	+	243	WP_000747951.1	hypothetical protein	-
ECOK1_RS07375	1439522..1439947	+	426	WP_000693867.1	Rha family transcriptional regulator	-
ECOK1_RS07380	1440019..1441089	+	1071	WP_001262357.1	hypothetical protein	-
ECOK1_RS07385	1441130..1441552	+	423	WP_001151216.1	DUF977 family protein	-
ECOK1_RS07390	1441744..1442706	+	963	WP_014639476.1	hypothetical protein	-
ECOK1_RS07395	1442722..1443723	+	1002	WP_000354966.1	hypothetical protein	-
-	1444271..1444329	-	59	-	-	Antitoxin
ECOK1_RS07405	1444341..1444496	+	156	WP_000813256.1	type I toxin-antitoxin system toxin HokD	Toxin
ECOK1_RS27740	1444664..1444942	+	279	WP_011478175.1	hypothetical protein	-
ECOK1_RS07415	1444944..1445990	+	1047	WP_001265108.1	DUF968 domain-containing protein	-
ECOK1_RS07420	1446003..1446377	+	375	WP_000904114.1	RusA family crossover junction endodeoxyribonuclease	-
ECOK1_RS07425	1446374..1447195	+	822	WP_000762880.1	antitermination protein	-
ECOK1_RS07430	1447420..1447617	+	198	WP_000917749.1	hypothetical protein	-
ECOK1_RS07435	1447768..1448817	+	1050	WP_000935515.1	site-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1428678..1481218	52540

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5764.94 Da
• Isoelectric Point: 6.1531

>T27229 WP_000813256.1 NC_017628:1444341-1444496 [Escherichia coli IHE3034]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTVYEPEE

Nucleotide

• Length: 156 bp

>T27229 NC_017628:1444341-1444496 [Escherichia coli IHE3034]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGTTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT27229 NC_017628:c1444329-1444271 [Escherichia coli IHE3034]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin