Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1128814..1129541 Replicon chromosome
Accession NZ_CP117720
Organism Escherichia coli strain B1W1

Toxin (Protein)


Gene name higB Uniprot ID J7Q991
Locus tag PTA75_RS05545 Protein ID WP_000547564.1
Coordinates 1128814..1129125 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag PTA75_RS05550 Protein ID WP_000126294.1
Coordinates 1129122..1129541 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PTA75_RS05515 (1123956) 1123956..1125665 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
PTA75_RS05520 (1125675) 1125675..1126217 + 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
PTA75_RS05525 (1126217) 1126217..1126984 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
PTA75_RS05530 (1126981) 1126981..1127391 + 411 WP_001291921.1 formate hydrogenlyase assembly protein HycH -
PTA75_RS05535 (1127384) 1127384..1127854 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
PTA75_RS05540 (1127879) 1127879..1128640 + 762 WP_001026446.1 hypothetical protein -
PTA75_RS05545 (1128814) 1128814..1129125 + 312 WP_000547564.1 type II toxin-antitoxin system HigB family toxin Toxin
PTA75_RS05550 (1129122) 1129122..1129541 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
PTA75_RS05555 (1129655) 1129655..1131079 - 1425 WP_000110363.1 6-phospho-beta-glucosidase AscB -
PTA75_RS05560 (1131088) 1131088..1132544 - 1457 Protein_1091 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
PTA75_RS05565 (1132804) 1132804..1133814 + 1011 WP_001392554.1 DNA-binding transcriptional regulator AscG -
PTA75_RS05570 (1133963) 1133963..1134490 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12426.13 Da        Isoelectric Point: 9.7248

>T272137 WP_000547564.1 NZ_CP117720:1128814-1129125 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT272137 WP_000126294.1 NZ_CP117720:1129122-1129541 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FJG5

Antitoxin

Source ID Structure

References