Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3803244..3803785 Replicon chromosome
Accession NZ_CP117714
Organism Escherichia coli strain PBS4

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag PTA50_RS18540 Protein ID WP_000615976.1
Coordinates 3803507..3803785 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag PTA50_RS18535 Protein ID WP_000729704.1
Coordinates 3803244..3803504 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PTA50_RS18510 (3798280) 3798280..3799419 + 1140 WP_000785662.1 flagellar type III secretion system protein FlhB -
PTA50_RS18515 (3799403) 3799403..3801496 + 2094 WP_001597626.1 flagellar biosynthesis protein FlhA -
PTA50_RS18520 (3801601) 3801601..3801879 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
PTA50_RS18525 (3801872) 3801872..3802228 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
PTA50_RS18530 (3802285) 3802285..3803058 - 774 WP_000543897.1 C40 family peptidase -
PTA50_RS18535 (3803244) 3803244..3803504 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
PTA50_RS18540 (3803507) 3803507..3803785 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
PTA50_RS18545 (3803941) 3803941..3804681 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
PTA50_RS18550 (3804652) 3804652..3805419 - 768 WP_000333380.1 class II glutamine amidotransferase -
PTA50_RS18555 (3805625) 3805625..3806203 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T272092 WP_000615976.1 NZ_CP117714:3803507-3803785 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT272092 WP_000729704.1 NZ_CP117714:3803244-3803504 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References