Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2355000..2355502 Replicon chromosome
Accession NC_017626
Organism Escherichia coli 042

Toxin (Protein)


Gene name yoeB Uniprot ID D3GZ85
Locus tag EC042_RS12115 Protein ID WP_000767820.1
Coordinates 2355000..2355254 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EC042_RS12120 Protein ID WP_001259255.1
Coordinates 2355251..2355502 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC042_RS12100 2351493..2352851 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EC042_RS30185 2352841..2352903 - 63 WP_010723108.1 membrane protein YoeI -
EC042_RS12105 2353118..2354047 - 930 WP_000803351.1 LysR family transcriptional regulator -
EC042_RS12110 2354093..2354917 - 825 WP_000754757.1 SDR family oxidoreductase -
EC042_RS12115 2355000..2355254 - 255 WP_000767820.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EC042_RS12120 2355251..2355502 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EC042_RS28060 2355786..2355836 + 51 WP_001364200.1 his operon leader peptide -
EC042_RS12125 2355982..2356881 + 900 WP_000131789.1 ATP phosphoribosyltransferase -
EC042_RS12130 2356887..2358191 + 1305 WP_001306412.1 histidinol dehydrogenase -
EC042_RS12135 2358188..2359258 + 1071 WP_000108970.1 histidinol-phosphate transaminase -
EC042_RS12140 2359258..2360325 + 1068 WP_000080071.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10182.64 Da        Isoelectric Point: 8.0353

>T27208 WP_000767820.1 NC_017626:c2355254-2355000 [Escherichia coli 042]
VKLIWSEESWDDYLYWQETDKRIVKKIIELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T27208 NC_017626:c2355254-2355000 [Escherichia coli 042]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CATTGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT27208 WP_001259255.1 NC_017626:c2355502-2355251 [Escherichia coli 042]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT27208 NC_017626:c2355502-2355251 [Escherichia coli 042]
ATGCGTACAATTAGCTACAGCGAAGCGCGCCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D3GZ85

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References