Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1681123..1681685 Replicon chromosome
Accession NC_017626
Organism Escherichia coli 042

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag EC042_RS08505 Protein ID WP_000605676.1
Coordinates 1681407..1681685 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag EC042_RS08500 Protein ID WP_000781370.1
Coordinates 1681123..1681407 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC042_RS08485 1676305..1679352 + 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -
EC042_RS08490 1679365..1680249 + 885 WP_001240565.1 formate dehydrogenase N subunit beta -
EC042_RS08495 1680242..1680895 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
EC042_RS08500 1681123..1681407 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
EC042_RS08505 1681407..1681685 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EC042_RS08510 1681871..1682881 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
EC042_RS08515 1683015..1684712 - 1698 WP_000433464.1 malate dehydrogenase -
EC042_RS08520 1684869..1685006 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
EC042_RS08525 1685108..1685323 - 216 WP_000495766.1 biofilm-dependent modulation protein -
EC042_RS08530 1685668..1686099 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T27197 WP_000605676.1 NC_017626:c1681685-1681407 [Escherichia coli 042]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T27197 NC_017626:c1681685-1681407 [Escherichia coli 042]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCATCGACTTGCTGTGATTGATGCGGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT27197 WP_000781370.1 NC_017626:c1681407-1681123 [Escherichia coli 042]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT27197 NC_017626:c1681407-1681123 [Escherichia coli 042]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGTCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGATTACGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References