Detailed information of TA system TA027192

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1535251..1535622
• Replicon: chromosome
• Accession: NC_017626
• Organism: Escherichia coli 042

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A839BBC2
• Locus tag: EC042_RS07750
• Protein ID: WP_021500490.1
• Coordinates: 1535428..1535622 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1535251..1535429 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC042_RS07725	1531206..1532579	+	1374	WP_000123753.1	ATP-dependent RNA helicase DbpA	-
EC042_RS07730	1532708..1533643	-	936	WP_001157377.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
EC042_RS27735	1533695..1534930	-	1236	WP_000040836.1	site-specific integrase	-
EC042_RS07740	1534932..1535147	-	216	WP_000079601.1	excisionase XisR	-
EC042_RS07745	1535247..1535435	-	189	WP_001383994.1	DUF1187 family protein	-
-	1535251..1535429	+	179	NuclAT_0	-	Antitoxin
-	1535251..1535429	+	179	NuclAT_0	-	Antitoxin
-	1535251..1535429	+	179	NuclAT_0	-	Antitoxin
-	1535251..1535429	+	179	NuclAT_0	-	Antitoxin
EC042_RS07750	1535428..1535622	-	195	WP_021500490.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
EC042_RS07755	1535686..1536774	-	1089	WP_001516464.1	recombinase RecT	-
EC042_RS07760	1536789..1539812	-	3024	WP_000105115.1	PD-(D/E)XK nuclease-like domain-containing protein	-
EC042_RS07765	1539914..1540189	-	276	WP_001516463.1	phage protein	-
EC042_RS07770	1540264..1540440	-	177	WP_000245522.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1533695..1579636	45941

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7046.98 Da
• Isoelectric Point: 9.2886

>T27192 WP_021500490.1 NC_017626:c1535622-1535428 [Escherichia coli 042]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T27192 NC_017626:c1535622-1535428 [Escherichia coli 042]
ATGCGGTATGAAAAAGTCAAACCATGCCCTTTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
CGCGAAGTGTAACGGATGCGAATCCCGAACCGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT27192 NC_017626:1535251-1535429 [Escherichia coli 042]
GACGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTGGTTAATTTTGTTTTTGAGTACCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A839BBC2

Antitoxin