Detailed information of TA system TA027183

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 313886..314536
• Replicon: chromosome
• Accession: NC_017626
• Organism: Escherichia coli 042

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: EC042_RS01480
• Protein ID: WP_000263532.1
• Coordinates: 313886..314227 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: D3GSB3
• Locus tag: EC042_RS01485
• Protein ID: WP_000212551.1
• Coordinates: 314237..314536 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC042_RS01455	308921..310021	+	1101	WP_001181877.1	flagellar basal body P-ring protein FlgI	-
EC042_RS01460	310021..310320	+	300	WP_000867281.1	rod-binding protein	-
EC042_RS01465	310496..311872	+	1377	WP_000367237.1	flagellar hook-associated protein FlgK	-
EC042_RS01470	311887..312816	+	930	WP_001266800.1	flagellar hook-associated protein FlgL	-
EC042_RS01475	312833..313810	+	978	WP_001195938.1	hypothetical protein	-
EC042_RS01480	313886..314227	+	342	WP_000263532.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EC042_RS01485	314237..314536	+	300	WP_000212551.1	XRE family transcriptional regulator	Antitoxin
EC042_RS01490	314594..315436	-	843	WP_000022366.1	winged helix-turn-helix domain-containing protein	-
EC042_RS01495	315922..316836	+	915	WP_000949083.1	lateral flagellin LafA	-
EC042_RS01505	317041..318357	+	1317	WP_000609678.1	flagellar filament capping protein FliD	-
EC042_RS01510	318380..318772	+	393	WP_000285319.1	flagellar export chaperone FliS	-
EC042_RS01515	318777..319088	+	312	WP_000415008.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	310496..325493	14997

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (8-88)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13031.86 Da
• Isoelectric Point: 6.4677

>T27183 WP_000263532.1 NC_017626:313886-314227 [Escherichia coli 042]
MWDVETTETFDNWFDAQTVALKEDLLAAMLILAEYGPQLGRPFADTVNDSQFSNMKELRVQHQGNPVRAFFAFDPARRGI
VLCAGDKTGLNEKKFYRDMIKLADSEYRKHLKK

Nucleotide

• Length: 342 bp

>T27183 NC_017626:313886-314227 [Escherichia coli 042]
ATGTGGGACGTCGAGACAACGGAGACTTTCGACAACTGGTTTGATGCCCAGACTGTTGCGCTGAAAGAAGATTTACTTGC
TGCAATGCTGATTCTTGCTGAGTATGGCCCACAGTTGGGCAGACCCTTTGCGGATACGGTTAATGATTCACAGTTTTCAA
ATATGAAAGAATTACGCGTCCAACATCAGGGAAACCCGGTACGTGCTTTTTTTGCTTTCGATCCTGCGCGCCGTGGCATT
GTATTATGTGCGGGTGATAAAACGGGGTTAAATGAGAAAAAGTTTTATCGCGATATGATCAAGCTCGCTGATTCTGAATA
CCGTAAGCATCTGAAAAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10976.81 Da
• Isoelectric Point: 9.7665

>AT27183 WP_000212551.1 NC_017626:314237-314536 [Escherichia coli 042]
MATLKELMAKQSPESQARIAAKVEEMRQTIALHQLREELNISQTEMAAAMGIKQPTLAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT27183 NC_017626:314237-314536 [Escherichia coli 042]
ATGGCTACTCTAAAAGAACTGATGGCAAAGCAAAGCCCTGAAAGCCAGGCGCGTATTGCGGCAAAAGTTGAGGAAATGCG
CCAGACCATCGCCTTGCATCAGCTGAGAGAAGAACTCAATATTTCGCAAACGGAAATGGCGGCAGCGATGGGCATTAAGC
AACCGACTCTTGCAAAAATGGAGCAGGCAGATAACGATCCTCGGTTGTCTACGCTAAAACGCTATGTTGCGGCTTTAGGT
GGGGAATTGAGTATCGACGTTAAACTGCCGAATGGTAAGAGAATTGCATTTCAGATTTAA

Structures

Antitoxin

• AlphaFold DB: D3GSB3