Detailed information of TA system TA271780

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 86861..87115
• Replicon: plasmid pEA22_1
• Accession: NZ_CP117629
• Organism: Escherichia albertii strain BIA_22

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: PS041_RS24870
• Protein ID: WP_001312851.1
• Coordinates: 86861..87010 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	87054..87115 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PS041_RS24825 (82358)	82358..82732	+	375	Protein_87	IS21 family transposase	-
PS041_RS24830 (82732)	82732..83231	+	500	Protein_88	ATP-binding protein	-
PS041_RS24835 (83229)	83229..83507	-	279	WP_001384452.1	type II toxin-antitoxin system toxin YacB	-
PS041_RS24840 (83507)	83507..83776	-	270	WP_273818329.1	type II toxin-antitoxin system antitoxin YacA	-
PS041_RS24845 (84689)	84689..85546	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
PS041_RS24850 (85539)	85539..86021	-	483	WP_001273588.1	hypothetical protein	-
PS041_RS24855 (86014)	86014..86061	-	48	WP_229471593.1	hypothetical protein	-
PS041_RS24860 (86052)	86052..86303	+	252	WP_223195197.1	replication protein RepA	-
PS041_RS24865 (86320)	86320..86577	-	258	WP_258141648.1	replication regulatory protein RepA	-
PS041_RS24870 (86861)	86861..87010	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (87054)	87054..87115	+	62	NuclAT_1	-	Antitoxin
- (87054)	87054..87115	+	62	NuclAT_1	-	Antitoxin
- (87054)	87054..87115	+	62	NuclAT_1	-	Antitoxin
- (87054)	87054..87115	+	62	NuclAT_1	-	Antitoxin
PS041_RS24875 (87468)	87468..89060	-	1593	WP_048237919.1	IS66 family transposase	-
PS041_RS24880 (89091)	89091..89441	-	351	WP_000624718.1	IS66 family insertion sequence element accessory protein TnpB	-
PS041_RS24885 (89438)	89438..89863	-	426	WP_000422741.1	transposase	-
PS041_RS24890 (90210)	90210..90422	-	213	WP_001299730.1	ANR family transcriptional regulator	-
PS041_RS24895 (90558)	90558..91118	-	561	WP_000139315.1	fertility inhibition protein FinO	-
PS041_RS24900 (91221)	91221..92081	-	861	WP_000704513.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	vat / vat / nleA/espI / espK / iutA / iucD / iucC / iucB / iucA	1..152064	152064

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T271780 WP_001312851.1 NZ_CP117629:c87010-86861 [Escherichia albertii]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T271780 NZ_CP117629:c87010-86861 [Escherichia albertii]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT271780 NZ_CP117629:87054-87115 [Escherichia albertii]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin