27176

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3624876..3625417 Replicon chromosome
Accession NC_017625
Organism Escherichia coli DH1

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag ECDH1_RS17985 Protein ID WP_000615983.1
Coordinates 3625139..3625417 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag ECDH1_RS17980 Protein ID WP_000729703.1
Coordinates 3624876..3625136 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECDH1_RS17960 3620551..3621336 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
ECDH1_RS17965 3621308..3623020 + 1713 Protein_3427 flagellar biosynthesis protein FlhA -
ECDH1_RS17970 3623244..3623741 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
ECDH1_RS17975 3623917..3624666 - 750 WP_000056849.1 C40 family peptidase -
ECDH1_RS17980 3624876..3625136 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
ECDH1_RS17985 3625139..3625417 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
ECDH1_RS17990 3625573..3626313 + 741 WP_001225679.1 murein L,D-transpeptidase -
ECDH1_RS17995 3626284..3627051 - 768 WP_000333380.1 class II glutamine amidotransferase -
ECDH1_RS18000 3627257..3627835 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T27176 WP_000615983.1 NC_017625:3625139-3625417 [Escherichia coli DH1]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T27176 NC_017625:3625139-3625417 [Escherichia coli DH1]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT27176 WP_000729703.1 NC_017625:3624876-3625136 [Escherichia coli DH1]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT27176 NC_017625:3624876-3625136 [Escherichia coli DH1]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
AlphaFold DB A0A5F1F716

References