Detailed information of TA system TA027175

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3618679..3619373
• Replicon: chromosome
• Accession: NC_017625
• Organism: Escherichia coli DH1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: ECDH1_RS17945
• Protein ID: WP_001263489.1
• Coordinates: 3618679..3619077 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: ECDH1_RS17950
• Protein ID: WP_000554758.1
• Coordinates: 3619080..3619373 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3614267..3614347	-	81	NuclAT_12	-	-
-	3614267..3614347	-	81	NuclAT_12	-	-
-	3614267..3614347	-	81	NuclAT_12	-	-
-	3614267..3614347	-	81	NuclAT_12	-	-
ECDH1_RS17920	3614943..3615401	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ECDH1_RS17925	3615662..3617119	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
ECDH1_RS17930	3617176..3617697	-	522	Protein_3420	peptide chain release factor H	-
ECDH1_RS17935	3617693..3617899	-	207	Protein_3421	RtcB family protein	-
ECDH1_RS17940	3618217..3618669	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
ECDH1_RS17945	3618679..3619077	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ECDH1_RS17950	3619080..3619373	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ECDH1_RS17955	3619425..3620480	-	1056	WP_001226164.1	DNA polymerase IV	-
ECDH1_RS17960	3620551..3621336	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
ECDH1_RS17965	3621308..3623020	+	1713	Protein_3427	flagellar biosynthesis protein FlhA	-
ECDH1_RS17970	3623244..3623741	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T27175 WP_001263489.1 NC_017625:c3619077-3618679 [Escherichia coli DH1]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T27175 NC_017625:c3619077-3618679 [Escherichia coli DH1]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT27175 WP_000554758.1 NC_017625:c3619373-3619080 [Escherichia coli DH1]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT27175 NC_017625:c3619373-3619080 [Escherichia coli DH1]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3