Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1788342..1788844 Replicon chromosome
Accession NC_017625
Organism Escherichia coli DH1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag ECDH1_RS08675 Protein ID WP_000767829.1
Coordinates 1788590..1788844 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag ECDH1_RS08670 Protein ID WP_001259255.1
Coordinates 1788342..1788593 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECDH1_RS08650 1783520..1784587 - 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
ECDH1_RS08655 1784587..1785657 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
ECDH1_RS08660 1785654..1786958 - 1305 WP_000009594.1 histidinol dehydrogenase -
ECDH1_RS08665 1786964..1787863 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
ECDH1_RS23670 1788009..1788059 - 51 WP_001364200.1 his operon leader peptide -
ECDH1_RS08670 1788342..1788593 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
ECDH1_RS08675 1788590..1788844 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
ECDH1_RS08680 1788927..1789751 + 825 WP_000754737.1 SDR family oxidoreductase -
ECDH1_RS08685 1789797..1790726 + 930 WP_000803351.1 LysR family transcriptional regulator -
ECDH1_RS25585 1790941..1791003 + 63 WP_010723108.1 membrane protein YoeI -
ECDH1_RS08690 1790993..1792351 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
ECDH1_RS08695 1792530..1793588 + 1059 WP_000492339.1 transport protein YeeE -
ECDH1_RS08700 1793602..1793829 + 228 WP_000234896.1 sulfurtransferase TusA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T27160 WP_000767829.1 NC_017625:1788590-1788844 [Escherichia coli DH1]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T27160 NC_017625:1788590-1788844 [Escherichia coli DH1]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT27160 WP_001259255.1 NC_017625:1788342-1788593 [Escherichia coli DH1]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT27160 NC_017625:1788342-1788593 [Escherichia coli DH1]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References