Detailed information of TA system TA027076

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-couple_hipB
• Location: 752069..752718
• Replicon: chromosome
• Accession: NC_017581
• Organism: Streptococcus thermophilus JIM 8232

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: STH8232_RS03955
• Protein ID: WP_014621406.1
• Coordinates: 752069..752365 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: F8LWH3
• Locus tag: STH8232_RS03960
• Protein ID: WP_014621407.1
• Coordinates: 752425..752718 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
STH8232_RS03935	747332..748468	+	1137	WP_014621402.1	abortive infection protein	-
STH8232_RS03940	748635..749390	+	756	WP_014621403.1	hypothetical protein	-
STH8232_RS10075	749932..750537	+	606	WP_082321157.1	restriction endonuclease subunit S	-
STH8232_RS03950	750554..751750	+	1197	WP_014621405.1	haloacid dehalogenase-like hydrolase	-
STH8232_RS03955	752069..752365	+	297	WP_014621406.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
STH8232_RS03960	752425..752718	+	294	WP_014621407.1	helix-turn-helix transcriptional regulator	Antitoxin
STH8232_RS03965	752740..754341	+	1602	WP_014621408.1	type I restriction-modification system subunit M	-
STH8232_RS11075	754458..754835	+	378	Protein_718	PD-(D/E)XK nuclease family transposase	-
STH8232_RS03975	754936..755936	+	1001	Protein_719	DNA/RNA non-specific endonuclease	-
STH8232_RS03980	756086..756646	+	561	WP_014621411.1	LemA family protein	-
STH8232_RS03985	756648..757547	+	900	WP_014621412.1	zinc metalloprotease HtpX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (36-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-88)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11779.67 Da
• Isoelectric Point: 10.2571

>T27076 WP_014621406.1 NC_017581:752069-752365 [Streptococcus thermophilus JIM 8232]
VHAIYFYKDKQGNQPVLDYMRELARRDSKDSRIKINKLNDYIELLSQHGTRAGQPYIKHLDAEIWELRLLRDRILFVAWL
DRSFVLLHPFVKKTQKTP

Nucleotide

• Length: 297 bp

>T27076 NC_017581:752069-752365 [Streptococcus thermophilus JIM 8232]
GTGCACGCTATTTATTTTTATAAGGATAAACAGGGGAATCAACCGGTCTTGGACTATATGAGGGAGCTAGCTCGTCGTGA
TAGCAAGGACAGCCGGATTAAAATCAACAAGCTCAACGACTATATTGAACTTTTGAGCCAGCATGGAACCCGTGCTGGCC
AACCCTATATCAAGCATCTGGATGCGGAGATCTGGGAGTTGAGACTCTTGCGGGATCGGATTTTATTCGTGGCTTGGCTG
GATAGGAGCTTTGTCTTACTGCATCCTTTTGTCAAAAAGACGCAAAAGACACCCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10744.50 Da
• Isoelectric Point: 5.8962

>AT27076 WP_014621407.1 NC_017581:752425-752718 [Streptococcus thermophilus JIM 8232]
MKNSAIGSNWKDVRAELFTKEEILESDLRVAIMTELIEARHEKGISQKKLEELSGVSQPVIARMETGKTSPQLDTVLKVL
ASLGKTLAVVPLEQEKI

Nucleotide

• Length: 294 bp

>AT27076 NC_017581:752425-752718 [Streptococcus thermophilus JIM 8232]
ATGAAGAATAGTGCCATCGGCAGCAATTGGAAAGATGTGAGAGCAGAGCTCTTCACCAAGGAAGAAATCCTGGAAAGTGA
TTTACGGGTCGCGATTATGACTGAGCTGATCGAGGCTCGACACGAAAAGGGCATTAGCCAAAAGAAACTAGAAGAACTCA
GTGGGGTCAGTCAACCGGTCATCGCTCGGATGGAGACAGGAAAGACCAGTCCTCAATTGGACACGGTTTTGAAAGTCTTA
GCCAGTCTGGGCAAGACCCTCGCTGTCGTCCCACTGGAACAAGAAAAGATCTAA

Structures

Antitoxin

• AlphaFold DB: F8LWH3