Detailed information of TA system TA027058

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 119399..120036
• Replicon: plasmid megaplasmid
• Accession: NC_017575
• Organism: Ralstonia solanacearum Po82

Toxin (Protein)

• Gene name: higB
• Uniprot ID: F6G785
• Locus tag: RSPO_RS16570
• Protein ID: WP_014618424.1
• Coordinates: 119399..119728 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RSPO_RS16575
• Protein ID: WP_003262799.1
• Coordinates: 119725..120036 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RSPO_RS16555	115108..116271	-	1164	WP_014618421.1	2-methylcitrate synthase	-
RSPO_RS16560	116309..117205	-	897	WP_014618422.1	methylisocitrate lyase	-
RSPO_RS16565	117399..119348	+	1950	WP_042548871.1	propionate catabolism operon regulatory protein PrpR	-
RSPO_RS16570	119399..119728	+	330	WP_014618424.1	hypothetical protein	Toxin
RSPO_RS16575	119725..120036	+	312	WP_003262799.1	transcriptional regulator	Antitoxin
RSPO_RS16580	120187..120495	-	309	WP_003262797.1	type II toxin-antitoxin system YafQ family toxin	-
RSPO_RS16585	120440..120751	-	312	WP_039551557.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
RSPO_RS25285	121124..121330	+	207	WP_014618427.1	hypothetical protein	-
RSPO_RS16590	121333..122166	-	834	WP_042548870.1	DUF2242 domain-containing protein	-
RSPO_RS16595	122463..122819	+	357	WP_042549721.1	hypothetical protein	-
RSPO_RS16600	123012..123752	-	741	WP_003262792.1	metallophosphatase family protein	-
RSPO_RS24265	123767..124081	+	315	WP_080773821.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cheY / cyaB / motA / cheW / cheB / cheY / adeG / acrB / fliI / fliM / flgG	1..1949172	1949172

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-91)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12413.89 Da
• Isoelectric Point: 5.2566

>T27058 WP_014618424.1 NC_017575:119399-119728 [Ralstonia solanacearum Po82]
MDATPIELPGEHDLSDDEYSALQQELLAQPDAGDVIKGTGGLRKLRFSDKRRGKGKRGGLRVIYYHWDGSSQFWMFVVYD
KDEAADLSSDERKVLARLLEQEVKARSER

Nucleotide

• Length: 330 bp

>T27058 NC_017575:119399-119728 [Ralstonia solanacearum Po82]
ATGGACGCTACCCCCATTGAGTTGCCGGGGGAGCACGATCTCTCGGACGACGAGTACAGCGCCCTTCAACAGGAACTGCT
CGCCCAGCCGGACGCTGGCGACGTCATCAAGGGGACAGGTGGTCTACGCAAGCTGCGGTTTTCGGACAAGCGGCGCGGCA
AAGGTAAGCGCGGCGGTCTGCGCGTCATCTATTACCACTGGGATGGAAGCAGCCAATTCTGGATGTTTGTCGTCTACGAC
AAAGACGAGGCGGCCGATCTTTCCAGTGACGAGCGCAAAGTGCTGGCACGGCTGTTGGAGCAGGAAGTCAAGGCAAGGAG
TGAGCGATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11620.59 Da
• Isoelectric Point: 10.3969

>AT27058 WP_003262799.1 NC_017575:119725-120036 [Ralstonia solanacearum Po82]
MKRNLFAELKEGMDALAAEREGKMTLRKVEVRVLQPIDVTAEEIKAVRAAAHASQAVMARRLRVNVRTYQNWEQGTAKPN
TQAAVLIKLVEKHPETLQMLEAL

Nucleotide

• Length: 312 bp

>AT27058 NC_017575:119725-120036 [Ralstonia solanacearum Po82]
ATGAAACGGAACCTGTTTGCTGAATTGAAGGAGGGGATGGACGCGCTGGCCGCAGAGCGCGAAGGAAAGATGACCCTCCG
CAAGGTCGAGGTCCGCGTGCTCCAGCCGATTGATGTCACCGCCGAGGAAATCAAGGCCGTTCGCGCAGCGGCCCATGCCT
CACAGGCTGTCATGGCACGTCGGTTGCGCGTCAATGTGCGCACCTACCAGAACTGGGAACAAGGCACCGCCAAACCGAAC
ACCCAGGCGGCCGTGTTGATCAAGCTCGTCGAGAAGCATCCGGAAACGTTGCAGATGCTGGAAGCGCTTTAA

Structures

Toxin

• AlphaFold DB: F6G785