Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3501644..3502314 Replicon chromosome
Accession NZ_CP117298
Organism Mycobacterium tuberculosis variant bovis strain OLF-AH-2022-TB-0122

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag PQK93_RS16525 Protein ID WP_003899954.1
Coordinates 3501644..3501988 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag PQK93_RS16530 Protein ID WP_003899955.1
Coordinates 3501985..3502314 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PQK93_RS16490 (PQK93_16490) 3496717..3497577 + 861 WP_003416628.1 alpha/beta hydrolase -
PQK93_RS16495 (PQK93_16495) 3497552..3498067 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
PQK93_RS16500 (PQK93_16500) 3498083..3498294 + 212 Protein_3257 (R)-hydratase -
PQK93_RS16505 (PQK93_16505) 3498307..3498600 + 294 WP_003416635.1 hypothetical protein -
PQK93_RS16510 (PQK93_16510) 3498888..3500177 + 1290 WP_003416640.1 ATP-binding protein -
PQK93_RS16515 (PQK93_16515) 3500524..3500958 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
PQK93_RS16520 (PQK93_16520) 3500961..3501413 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
PQK93_RS16525 (PQK93_16525) 3501644..3501988 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PQK93_RS16530 (PQK93_16530) 3501985..3502314 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
PQK93_RS16535 (PQK93_16535) 3502852..3503199 + 348 WP_003899956.1 DUF2384 domain-containing protein -
PQK93_RS16540 (PQK93_16540) 3503196..3503816 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
PQK93_RS16545 (PQK93_16545) 3503976..3505241 - 1266 WP_003909837.1 hypothetical protein -
PQK93_RS16550 (PQK93_16550) 3505409..3505618 + 210 WP_003416778.1 hypothetical protein -
PQK93_RS16555 (PQK93_16555) 3505865..3506899 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T270370 WP_003899954.1 NZ_CP117298:3501644-3501988 [Mycobacterium tuberculosis variant bovis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT270370 WP_003899955.1 NZ_CP117298:3501985-3502314 [Mycobacterium tuberculosis variant bovis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References