Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2238945..2239864 Replicon chromosome
Accession NZ_CP117298
Organism Mycobacterium tuberculosis variant bovis strain OLF-AH-2022-TB-0122

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag PQK93_RS10495 Protein ID WP_003900449.1
Coordinates 2239259..2239864 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag PQK93_RS10490 Protein ID WP_003410124.1
Coordinates 2238945..2239250 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PQK93_RS10460 (PQK93_10460) 2233956..2235212 - 1257 WP_003410095.1 HNH endonuclease signature motif containing protein -
PQK93_RS10465 (PQK93_10465) 2235566..2236141 + 576 WP_003410100.1 hypothetical protein -
PQK93_RS10470 (PQK93_10470) 2236138..2237178 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
PQK93_RS10475 (PQK93_10475) 2237420..2238139 + 720 WP_010950643.1 DUF433 domain-containing protein -
PQK93_RS10480 (PQK93_10480) 2238129..2238545 + 417 WP_003410114.1 hypothetical protein -
PQK93_RS10485 (PQK93_10485) 2238561..2238860 - 300 WP_003410120.1 hypothetical protein -
PQK93_RS10490 (PQK93_10490) 2238945..2239250 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
PQK93_RS10495 (PQK93_10495) 2239259..2239864 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PQK93_RS10500 (PQK93_10500) 2239889..2240248 - 360 WP_003410131.1 hypothetical protein -
PQK93_RS10505 (PQK93_10505) 2240408..2241061 - 654 WP_014324107.1 hypothetical protein -
PQK93_RS10510 (PQK93_10510) 2241037..2241273 - 237 WP_003410133.1 hypothetical protein -
PQK93_RS10515 (PQK93_10515) 2241480..2242376 - 897 WP_003410136.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T270353 WP_003900449.1 NZ_CP117298:c2239864-2239259 [Mycobacterium tuberculosis variant bovis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT270353 WP_003410124.1 NZ_CP117298:c2239250-2238945 [Mycobacterium tuberculosis variant bovis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References