Detailed information of TA system TA027026

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 178980..179503
• Replicon: chromosome
• Accession: NC_017568
• Organism: Staphylococcus pseudintermedius ED99

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A8H9EQJ3
• Locus tag: SPSE_RS00765
• Protein ID: WP_014612815.1
• Coordinates: 179237..179503 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A3D8YKM0
• Locus tag: SPSE_RS00760
• Protein ID: WP_014612814.1
• Coordinates: 178980..179237 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SPSE_RS00740	174327..174509	-	183	WP_041613203.1	hypothetical protein	-
SPSE_RS00745	175035..175841	+	807	WP_014612811.1	arylamine N-acetyltransferase	-
SPSE_RS00750	176135..176629	-	495	WP_014612812.1	membrane protein	-
SPSE_RS00755	176882..178786	+	1905	WP_014612813.1	triacylglycerol lipase	-
SPSE_RS00760	178980..179237	+	258	WP_014612814.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
SPSE_RS00765	179237..179503	+	267	WP_014612815.1	Txe/YoeB family addiction module toxin	Toxin
SPSE_RS12060	179864..179965	-	102	WP_019169745.1	DUF2648 domain-containing protein	-
SPSE_RS00770	179979..181472	-	1494	WP_014612817.1	malate dehydrogenase (quinone)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-68)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10555.15 Da
• Isoelectric Point: 10.2281

>T27026 WP_014612815.1 NC_017568:179237-179503 [Staphylococcus pseudintermedius ED99]
MGNYSVLIKNSAKNDLKKIKKSLLKEQFSKIIEVLKINPYEASQSFEKLQPKHSARYLRRLNHQHRVVYTVDDEKREVYI
FSAWSHYE

Nucleotide

• Length: 267 bp

>T27026 NC_017568:179237-179503 [Staphylococcus pseudintermedius ED99]
ATGGGTAATTATTCTGTTTTAATTAAGAATTCAGCCAAAAATGACTTAAAAAAAATTAAAAAGTCACTTCTTAAAGAGCA
ATTTTCAAAAATTATTGAAGTTTTAAAGATTAACCCTTATGAAGCTAGTCAATCTTTTGAGAAGCTACAACCGAAGCATT
CAGCTCGATATTTAAGGCGTCTGAATCATCAACATAGAGTTGTGTATACGGTTGATGATGAAAAAAGAGAAGTTTATATT
TTTTCTGCTTGGTCACATTATGAGTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9866.87 Da
• Isoelectric Point: 4.1100

>AT27026 WP_014612814.1 NC_017568:178980-179237 [Staphylococcus pseudintermedius ED99]
MIIKTPTNARKEFYELLKQVNDNHEPIIISGSKSENNAVIVSQEDWDSIQETLFLEYTGTMDKVRAREKDDSGYTNIDDI
DWDNL

Nucleotide

• Length: 258 bp

>AT27026 NC_017568:178980-179237 [Staphylococcus pseudintermedius ED99]
ATGATTATTAAAACGCCTACAAATGCTAGAAAAGAGTTTTACGAGTTGTTAAAACAAGTTAATGATAATCATGAGCCTAT
TATAATAAGTGGTTCTAAAAGTGAAAACAATGCAGTTATTGTTAGTCAGGAAGACTGGGATAGTATTCAAGAAACGTTAT
TTCTCGAATACACTGGTACTATGGATAAAGTAAGAGCACGTGAAAAAGATGATAGTGGTTATACCAATATTGACGATATA
GATTGGGATAATTTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3D8YKM0