Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2595099..2595661 Replicon chromosome
Accession NZ_CP117235
Organism Escherichia coli strain ATCC 25922

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag PQQ28_RS12750 Protein ID WP_000605675.1
Coordinates 2595099..2595377 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag PQQ28_RS12755 Protein ID WP_000781370.1
Coordinates 2595377..2595661 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PQQ28_RS12725 (2590685) 2590685..2591116 - 432 WP_000152307.1 peroxiredoxin OsmC -
PQQ28_RS12730 (2591461) 2591461..2591676 + 216 WP_000495766.1 biofilm-dependent modulation protein -
PQQ28_RS12735 (2591778) 2591778..2591915 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
PQQ28_RS12740 (2592072) 2592072..2593769 + 1698 WP_000433460.1 malate dehydrogenase -
PQQ28_RS12745 (2593903) 2593903..2594913 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
PQQ28_RS12750 (2595099) 2595099..2595377 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PQQ28_RS12755 (2595377) 2595377..2595661 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
PQQ28_RS12760 (2595890) 2595890..2596543 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
PQQ28_RS12765 (2596536) 2596536..2597420 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
PQQ28_RS12770 (2597433) 2597433..2600480 - 3048 WP_011076399.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T270135 WP_000605675.1 NZ_CP117235:2595099-2595377 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT270135 WP_000781370.1 NZ_CP117235:2595377-2595661 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References