Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1213121..1213848 Replicon chromosome
Accession NZ_CP117235
Organism Escherichia coli strain ATCC 25922

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag PQQ28_RS06255 Protein ID WP_000547555.1
Coordinates 1213121..1213432 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H2V9Z2
Locus tag PQQ28_RS06260 Protein ID WP_000126293.1
Coordinates 1213429..1213848 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PQQ28_RS06225 (1208262) 1208262..1209971 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
PQQ28_RS06230 (1209981) 1209981..1210523 + 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
PQQ28_RS06235 (1210523) 1210523..1211290 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
PQQ28_RS06240 (1211287) 1211287..1211697 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
PQQ28_RS06245 (1211690) 1211690..1212160 + 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
PQQ28_RS06250 (1212203) 1212203..1212958 + 756 WP_001305268.1 hypothetical protein -
PQQ28_RS06255 (1213121) 1213121..1213432 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
PQQ28_RS06260 (1213429) 1213429..1213848 + 420 WP_000126293.1 helix-turn-helix domain-containing protein Antitoxin
PQQ28_RS06265 (1213940) 1213940..1214368 - 429 WP_000536064.1 hypothetical protein -
PQQ28_RS06270 (1214753) 1214753..1215280 + 528 WP_001078777.1 electron transport protein HydN -
PQQ28_RS06275 (1215433) 1215433..1217685 + 2253 WP_001107659.1 carbamoyltransferase HypF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T270127 WP_000547555.1 NZ_CP117235:1213121-1213432 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15426.46 Da        Isoelectric Point: 4.4596

>AT270127 WP_000126293.1 NZ_CP117235:1213429-1213848 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2V9Z2

References