Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2300..2946 Replicon plasmid pATCC25922_4
Accession NZ_CP117232
Organism Escherichia coli strain ATCC 25922

Toxin (Protein)


Gene name higB Uniprot ID A0A7R6ZH56
Locus tag PQQ28_RS00020 Protein ID WP_000269913.1
Coordinates 2300..2647 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1V3UZT0
Locus tag PQQ28_RS00025 Protein ID WP_001259436.1
Coordinates 2647..2946 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PQQ28_RS00010 (PQQ28_00010) 1481..1768 + 288 WP_000356589.1 hypothetical protein -
PQQ28_RS00015 (PQQ28_00015) 1792..2055 + 264 WP_000424604.1 hypothetical protein -
PQQ28_RS00020 (PQQ28_00020) 2300..2647 + 348 WP_000269913.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PQQ28_RS00025 (PQQ28_00025) 2647..2946 + 300 WP_001259436.1 XRE family transcriptional regulator Antitoxin
PQQ28_RS00030 (PQQ28_00030) 3110..3490 + 381 WP_000061764.1 hypothetical protein -
PQQ28_RS00035 (PQQ28_00035) 3554..3826 + 273 WP_000148349.1 helix-turn-helix domain-containing protein -
PQQ28_RS00040 (PQQ28_00040) 4437..4625 + 189 WP_000986262.1 hypothetical protein -
PQQ28_RS00045 (PQQ28_00045) 4636..5778 - 1143 WP_000854799.1 ORF6N domain-containing protein -
PQQ28_RS00050 (PQQ28_00050) 5775..5966 - 192 WP_000183352.1 hypothetical protein -
PQQ28_RS00055 (PQQ28_00055) 6540..7112 + 573 WP_000137931.1 helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..48488 48488


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-105)

Antitoxin

Cro/C1-type helix-turn-helix domain

(32-80)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13486.53 Da        Isoelectric Point: 9.8723

>T270123 WP_000269913.1 NZ_CP117232:2300-2647 [Escherichia coli]
MWTVLFSQRFDGWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYKKLVRIAEDEFAAHLNTLESK

Download         Length: 348 bp

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11074.92 Da        Isoelectric Point: 5.2090

>AT270123 WP_001259436.1 NZ_CP117232:2647-2946 [Escherichia coli]
MRTLDEVIASRSPESQARIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRIAFNI

Download         Length: 300 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7R6ZH56

Antitoxin

Source ID Structure
AlphaFold DB A0A1V3UZT0

References