Detailed information of TA system TA270080

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 132080..132731
• Replicon: plasmid unnamed2
• Accession: NZ_CP117201
• Organism: Pantoea sp. SS70

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: PO881_RS23730
• Protein ID: WP_094111544.1
• Coordinates: 132080..132424 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PO881_RS23735
• Protein ID: WP_280173356.1
• Coordinates: 132417..132731 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PO881_RS23705 (PO881_23705)	128382..128816	+	435	WP_280173352.1	antirestriction protein	-
PO881_RS23710 (PO881_23710)	128860..129237	+	378	WP_280173353.1	DUF1380 family protein	-
PO881_RS23715 (PO881_23715)	130217..130735	+	519	WP_280173488.1	JAB domain-containing protein	-
PO881_RS23720 (PO881_23720)	130831..131277	+	447	WP_280173354.1	conjugation system SOS inhibitor PsiB family protein	-
PO881_RS23725 (PO881_23725)	131274..132026	+	753	WP_280173355.1	plasmid SOS inhibition protein A	-
PO881_RS23730 (PO881_23730)	132080..132424	+	345	WP_094111544.1	toxin	Toxin
PO881_RS23735 (PO881_23735)	132417..132731	+	315	WP_280173356.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
PO881_RS23740 (PO881_23740)	132810..133208	+	399	WP_280173357.1	hypothetical protein	-
PO881_RS23745 (PO881_23745)	133685..134422	+	738	WP_280173358.1	DUF905 family protein	-
PO881_RS23750 (PO881_23750)	134507..134656	+	150	WP_280173359.1	succinate dehydrogenase flavoprotein subunit	-
PO881_RS23755 (PO881_23755)	134653..135225	+	573	WP_280173360.1	hypothetical protein	-
PO881_RS23760 (PO881_23760)	135316..135642	+	327	WP_280173361.1	hypothetical protein	-
PO881_RS23765 (PO881_23765)	135647..135838	+	192	WP_280173362.1	hypothetical protein	-
PO881_RS23770 (PO881_23770)	136553..136813	+	261	WP_280173363.1	hypothetical protein	-
PO881_RS23775 (PO881_23775)	136912..137160	+	249	WP_222235171.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..196862	196862

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Antitoxin MqsA/HigA-2 (19-99)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13515.30 Da
• Isoelectric Point: 9.6740

>T270080 WP_094111544.1 NZ_CP117201:132080-132424 [Pantoea sp. SS70]
MHATFIELSSFQKYRADYLNDDQFRLFQNMLLADPEKGDVIPDTGGLRKVRFRDERRNKGTRGGIRVIYYWTNEKGQFIL
FTIYDKDQRDDLTKQQRDALAEALNVIKKGLRHD

Nucleotide

• Length: 345 bp

>T270080 NZ_CP117201:132080-132424 [Pantoea sp. SS70]
ATGCACGCGACATTTATTGAGCTTTCCAGCTTTCAGAAATACCGGGCTGACTACCTGAACGACGATCAGTTCAGGTTGTT
TCAGAACATGTTGCTGGCTGACCCCGAAAAGGGCGACGTGATCCCGGATACGGGTGGCCTGCGTAAAGTCCGGTTCAGGG
ACGAGCGCCGGAACAAAGGCACACGAGGCGGGATCAGGGTTATCTATTACTGGACGAACGAAAAAGGCCAGTTCATCCTG
TTTACCATCTACGATAAGGATCAACGCGACGATCTGACCAAACAGCAGCGCGACGCCCTGGCGGAGGCGCTTAACGTGAT
TAAAAAGGGGCTGAGACATGACTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12053.77 Da
• Isoelectric Point: 7.3176

>AT270080 WP_280173356.1 NZ_CP117201:132417-132731 [Pantoea sp. SS70]
MTERDIFSELMTGMEELKDHQDGKITLKTYKVSKRAPVTIEPQELRDVREKLNLSQAVFAHYLHTGETTYQNWEQGRAKP
NAQAVLLIRMVQQNPDTLRTLAQL

Nucleotide

• Length: 315 bp

>AT270080 NZ_CP117201:132417-132731 [Pantoea sp. SS70]
ATGACTGAACGTGACATTTTCAGTGAGCTGATGACTGGCATGGAGGAGCTGAAAGATCACCAGGACGGGAAAATCACGCT
CAAGACTTACAAGGTCAGCAAGCGCGCACCCGTCACTATCGAGCCGCAGGAGCTGCGCGACGTGCGCGAAAAACTTAACC
TGTCACAGGCGGTTTTCGCACACTATCTGCATACGGGCGAGACCACCTATCAGAACTGGGAGCAGGGCAGAGCTAAGCCT
AACGCTCAGGCCGTCCTGCTGATCCGCATGGTTCAGCAGAACCCGGACACGCTGCGGACGCTCGCGCAGCTGTAA