Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3875127..3875668 Replicon chromosome
Accession NZ_CP117053
Organism Escherichia coli strain MLI106K4

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag NL407_RS20025 Protein ID WP_000615983.1
Coordinates 3875390..3875668 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag NL407_RS20020 Protein ID WP_000729703.1
Coordinates 3875127..3875387 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL407_RS20000 (3870802) 3870802..3871587 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
NL407_RS20005 (3871559) 3871559..3873271 + 1713 Protein_3696 flagellar biosynthesis protein FlhA -
NL407_RS20010 (3873495) 3873495..3873992 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
NL407_RS20015 (3874168) 3874168..3874917 - 750 WP_000056849.1 C40 family peptidase -
NL407_RS20020 (3875127) 3875127..3875387 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
NL407_RS20025 (3875390) 3875390..3875668 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
NL407_RS20030 (3875824) 3875824..3876564 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
NL407_RS20035 (3876535) 3876535..3877302 - 768 WP_000333380.1 class II glutamine amidotransferase -
NL407_RS20040 (3877508) 3877508..3878086 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3850825..3884210 33385


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T269969 WP_000615983.1 NZ_CP117053:3875390-3875668 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT269969 WP_000729703.1 NZ_CP117053:3875127-3875387 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References