Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3868930..3869624 Replicon chromosome
Accession NZ_CP117049
Organism Escherichia coli strain MLI106K2

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag NL423_RS19950 Protein ID WP_001263489.1
Coordinates 3868930..3869328 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag NL423_RS19955 Protein ID WP_000554758.1
Coordinates 3869331..3869624 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3864518) 3864518..3864598 - 81 NuclAT_13 - -
- (3864518) 3864518..3864598 - 81 NuclAT_13 - -
- (3864518) 3864518..3864598 - 81 NuclAT_13 - -
- (3864518) 3864518..3864598 - 81 NuclAT_13 - -
NL423_RS19925 (3865194) 3865194..3865652 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
NL423_RS19930 (3865913) 3865913..3867370 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
NL423_RS19935 (3867427) 3867427..3867948 - 522 Protein_3684 peptide chain release factor H -
NL423_RS19940 (3867944) 3867944..3868150 - 207 Protein_3685 RtcB family protein -
NL423_RS19945 (3868468) 3868468..3868920 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
NL423_RS19950 (3868930) 3868930..3869328 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
NL423_RS19955 (3869331) 3869331..3869624 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
NL423_RS19960 (3869676) 3869676..3870731 - 1056 WP_001226164.1 DNA polymerase IV -
NL423_RS19965 (3870802) 3870802..3871587 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
NL423_RS19970 (3871559) 3871559..3873271 + 1713 Protein_3691 flagellar biosynthesis protein FlhA -
NL423_RS19975 (3873495) 3873495..3873992 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3850825..3884210 33385


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T269935 WP_001263489.1 NZ_CP117049:c3869328-3868930 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT269935 WP_000554758.1 NZ_CP117049:c3869624-3869331 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References