Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 608317..609116 Replicon chromosome
Accession NZ_CP117049
Organism Escherichia coli strain MLI106K2

Toxin (Protein)


Gene name yhaV Uniprot ID D3GWU5
Locus tag NL423_RS04080 Protein ID WP_000347272.1
Coordinates 608317..608781 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag NL423_RS04085 Protein ID WP_001307405.1
Coordinates 608781..609116 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL423_RS04050 (603318) 603318..603752 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
NL423_RS04055 (603770) 603770..604648 - 879 WP_001298314.1 PTS N-acetylgalactosamine transporter subunit IID -
NL423_RS04060 (604638) 604638..605417 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
NL423_RS04065 (605428) 605428..605901 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
NL423_RS04070 (605924) 605924..607204 - 1281 WP_001521382.1 tagatose-bisphosphate aldolase subunit KbaZ -
NL423_RS04075 (607453) 607453..608262 + 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
NL423_RS04080 (608317) 608317..608781 - 465 WP_000347272.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
NL423_RS04085 (608781) 608781..609116 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
NL423_RS04090 (609265) 609265..610836 - 1572 WP_001273738.1 galactarate dehydratase -
NL423_RS04095 (611211) 611211..612545 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
NL423_RS04100 (612561) 612561..613331 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17832.26 Da        Isoelectric Point: 9.6924

>T269920 WP_000347272.1 NZ_CP117049:c608781-608317 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT269920 WP_001307405.1 NZ_CP117049:c609116-608781 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829KUD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References