Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 3276320..3276922 Replicon chromosome
Accession NZ_CP117020
Organism Escherichia coli strain MLI106K3

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag NL424_RS16135 Protein ID WP_000897305.1
Coordinates 3276320..3276631 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NL424_RS16140 Protein ID WP_000356395.1
Coordinates 3276632..3276922 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL424_RS16110 (3271362) 3271362..3271799 + 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
NL424_RS16115 (3271844) 3271844..3272785 + 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
NL424_RS16120 (3273201) 3273201..3274088 + 888 Protein_3077 hypothetical protein -
NL424_RS16125 (3274101) 3274101..3275015 + 915 WP_109553727.1 transposase -
NL424_RS16130 (3275183) 3275183..3276091 - 909 WP_001550353.1 alpha/beta hydrolase -
NL424_RS16135 (3276320) 3276320..3276631 + 312 WP_000897305.1 hypothetical protein Toxin
NL424_RS16140 (3276632) 3276632..3276922 + 291 WP_000356395.1 NadS family protein Antitoxin
NL424_RS16145 (3277007) 3277007..3277228 + 222 WP_001550354.1 hypothetical protein -
NL424_RS16150 (3277280) 3277280..3277558 + 279 WP_001315112.1 hypothetical protein -
NL424_RS16155 (3277977) 3277977..3278195 + 219 WP_001295676.1 CopG family transcriptional regulator -
NL424_RS16160 (3278414) 3278414..3278656 + 243 WP_001309881.1 CopG family transcriptional regulator -
NL424_RS16165 (3278838) 3278838..3279767 - 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
NL424_RS16170 (3279764) 3279764..3280399 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
NL424_RS16175 (3280396) 3280396..3281298 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T269865 WP_000897305.1 NZ_CP117020:3276320-3276631 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10869.46 Da        Isoelectric Point: 10.8907

>AT269865 WP_000356395.1 NZ_CP117020:3276632-3276922 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References