Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2440456..2441255 Replicon chromosome
Accession NZ_CP117020
Organism Escherichia coli strain MLI106K3

Toxin (Protein)


Gene name yhaV Uniprot ID F0JQM9
Locus tag NL424_RS12145 Protein ID WP_000347270.1
Coordinates 2440791..2441255 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A0V9H1L4
Locus tag NL424_RS12140 Protein ID WP_001551693.1
Coordinates 2440456..2440791 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL424_RS12125 (2436241) 2436241..2437011 - 771 WP_001551692.1 2-dehydro-3-deoxyglucarate aldolase -
NL424_RS12130 (2437027) 2437027..2438361 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
NL424_RS12135 (2438736) 2438736..2440307 + 1572 WP_001273738.1 galactarate dehydratase -
NL424_RS12140 (2440456) 2440456..2440791 + 336 WP_001551693.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
NL424_RS12145 (2440791) 2440791..2441255 + 465 WP_000347270.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
NL424_RS12150 (2441310) 2441310..2442119 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
NL424_RS12155 (2442368) 2442368..2443648 + 1281 WP_001551694.1 tagatose-bisphosphate aldolase subunit KbaZ -
NL424_RS12160 (2443671) 2443671..2444144 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
NL424_RS12165 (2444155) 2444155..2444934 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
NL424_RS12170 (2444924) 2444924..2445802 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
NL424_RS12175 (2445820) 2445820..2446254 + 435 WP_000948837.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2431694..2441255 9561


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T269864 WP_000347270.1 NZ_CP117020:2440791-2441255 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12347.87 Da        Isoelectric Point: 4.6890

>AT269864 WP_001551693.1 NZ_CP117020:2440456-2440791 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NQ90

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9H1L4

References