Detailed information of TA system TA026985

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1001403..1001998
• Replicon: chromosome
• Accession: NC_017549
• Organism: Pseudomonas aeruginosa NCGM2.S1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: NCGM2_RS31690
• Protein ID: WP_003113526.1
• Coordinates: 1001403..1001681 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NCGM2_RS04485
• Protein ID: WP_003133769.1
• Coordinates: 1001693..1001998 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NCGM2_RS04470	996828..998066	+	1239	WP_003095023.1	C69 family dipeptidase	-
NCGM2_RS04475	998128..998775	+	648	WP_003095021.1	carbonate dehydratase	-
NCGM2_RS04480	998846..1001074	-	2229	WP_023104079.1	TonB-dependent receptor	-
NCGM2_RS31690	1001403..1001681	+	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
NCGM2_RS04485	1001693..1001998	+	306	WP_003133769.1	HigA family addiction module antidote protein	Antitoxin
NCGM2_RS04495	1002393..1003493	-	1101	WP_003095017.1	redox-regulated ATPase YchF	-
NCGM2_RS04500	1003534..1004118	-	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
NCGM2_RS04505	1004160..1004774	-	615	WP_003095013.1	50S ribosomal protein L25/general stress protein Ctc	-
NCGM2_RS04510	1004891..1005832	-	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
NCGM2_RS04520	1005999..1006847	-	849	WP_003095007.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T26985 WP_003113526.1 NC_017549:1001403-1001681 [Pseudomonas aeruginosa NCGM2.S1]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T26985 NC_017549:1001403-1001681 [Pseudomonas aeruginosa NCGM2.S1]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT26985 WP_003133769.1 NC_017549:1001693-1001998 [Pseudomonas aeruginosa NCGM2.S1]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT26985 NC_017549:1001693-1001998 [Pseudomonas aeruginosa NCGM2.S1]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAATGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3