Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2308346..2309145 Replicon chromosome
Accession NZ_CP117016
Organism Escherichia coli strain MLI121

Toxin (Protein)


Gene name yhaV Uniprot ID A0A7B2BI69
Locus tag NL415_RS12060 Protein ID WP_000347258.1
Coordinates 2308681..2309145 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A368IY36
Locus tag NL415_RS12055 Protein ID WP_001604880.1
Coordinates 2308346..2308681 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL415_RS12040 (2304131) 2304131..2304901 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
NL415_RS12045 (2304917) 2304917..2306251 - 1335 WP_000599654.1 galactarate/glucarate/glycerate transporter GarP -
NL415_RS12050 (2306626) 2306626..2308197 + 1572 WP_001273737.1 galactarate dehydratase -
NL415_RS12055 (2308346) 2308346..2308681 + 336 WP_001604880.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
NL415_RS12060 (2308681) 2308681..2309145 + 465 WP_000347258.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
NL415_RS12065 (2309200) 2309200..2310009 - 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
NL415_RS12070 (2310258) 2310258..2311538 + 1281 WP_000681943.1 tagatose-bisphosphate aldolase subunit KbaZ -
NL415_RS12075 (2311561) 2311561..2312034 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
NL415_RS12080 (2312045) 2312045..2312824 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
NL415_RS12085 (2312814) 2312814..2313692 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
NL415_RS12090 (2313710) 2313710..2314144 + 435 WP_000948829.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2299104..2309145 10041


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17844.32 Da        Isoelectric Point: 9.6924

>T269836 WP_000347258.1 NZ_CP117016:2308681-2309145 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEILKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12340.91 Da        Isoelectric Point: 4.8616

>AT269836 WP_001604880.1 NZ_CP117016:2308346-2308681 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGLDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7B2BI69

Antitoxin

Source ID Structure
AlphaFold DB A0A368IY36

References