Detailed information of TA system TA026983

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5295159..5295754
• Replicon: chromosome
• Accession: NC_017548
• Organism: Pseudomonas aeruginosa M18

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: PAM18_RS30080
• Protein ID: WP_003117425.1
• Coordinates: 5295476..5295754 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PAM18_RS24455
• Protein ID: WP_003113527.1
• Coordinates: 5295159..5295464 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PAM18_RS30065	5290243..5290530	-	288	WP_079262959.1	DUF5447 family protein	-
PAM18_RS24445	5290706..5290978	-	273	WP_004352675.1	hypothetical protein	-
PAM18_RS30075	5291425..5291760	+	336	WP_079262960.1	hypothetical protein	-
PAM18_RS30380	5291772..5292443	-	672	WP_125075348.1	hypothetical protein	-
PAM18_RS30385	5292445..5293572	-	1128	WP_125075349.1	hypothetical protein	-
PAM18_RS30390	5293565..5294542	-	978	WP_125080763.1	hypothetical protein	-
PAM18_RS24455	5295159..5295464	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
PAM18_RS30080	5295476..5295754	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PAM18_RS24460	5296083..5298311	+	2229	WP_003113525.1	TonB-dependent receptor	-
PAM18_RS24465	5298381..5299028	-	648	WP_003095021.1	carbonate dehydratase	-
PAM18_RS24470	5299090..5300328	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T26983 WP_003117425.1 NC_017548:c5295754-5295476 [Pseudomonas aeruginosa M18]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T26983 NC_017548:c5295754-5295476 [Pseudomonas aeruginosa M18]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT26983 WP_003113527.1 NC_017548:c5295464-5295159 [Pseudomonas aeruginosa M18]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT26983 NC_017548:c5295464-5295159 [Pseudomonas aeruginosa M18]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5