Detailed information of TA system TA026982

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4658643..4659324
• Replicon: chromosome
• Accession: NC_017548
• Organism: Pseudomonas aeruginosa M18

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6AKP0
• Locus tag: PAM18_RS21540
• Protein ID: WP_003111825.1
• Coordinates: 4658959..4659324 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1C7BMJ2
• Locus tag: PAM18_RS21535
• Protein ID: WP_003159602.1
• Coordinates: 4658643..4658966 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PAM18_RS21510	4654602..4655285	-	684	WP_003120766.1	TetR/AcrR family transcriptional regulator	-
PAM18_RS21515	4655373..4656251	+	879	WP_014603214.1	hypothetical protein	-
PAM18_RS29990	4656854..4657081	+	228	WP_014603215.1	hypothetical protein	-
PAM18_RS29995	4657096..4657638	-	543	WP_019396018.1	tyrosine-type recombinase/integrase	-
PAM18_RS30000	4657746..4658021	-	276	WP_004353146.1	hypothetical protein	-
PAM18_RS21525	4658021..4658353	-	333	WP_021205399.1	hypothetical protein	-
PAM18_RS30360	4658408..4658527	+	120	Protein_4288	IS5/IS1182 family transposase	-
PAM18_RS21535	4658643..4658966	-	324	WP_003159602.1	XRE family transcriptional regulator	Antitoxin
PAM18_RS21540	4658959..4659324	-	366	WP_003111825.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PAM18_RS30485	4659615..4659983	-	369	WP_162471085.1	hypothetical protein	-
PAM18_RS21545	4659996..4660268	+	273	WP_014603216.1	hypothetical protein	-
PAM18_RS21550	4660298..4660723	-	426	WP_003116492.1	VOC family protein	-
PAM18_RS21555	4660824..4661708	+	885	WP_003085663.1	LysR family transcriptional regulator	-
PAM18_RS21560	4661681..4662634	-	954	WP_003085661.1	LysR family transcriptional regulator	-
PAM18_RS21565	4662855..4663289	+	435	WP_003116494.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13880.24 Da
• Isoelectric Point: 4.8219

>T26982 WP_003111825.1 NC_017548:c4659324-4658959 [Pseudomonas aeruginosa M18]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRHGHMRELRTQHGGRPFRTLYAFDPRRSA
ILLIGGDKTGDDRWYELNVPIADRLYDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T26982 NC_017548:c4659324-4658959 [Pseudomonas aeruginosa M18]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCATG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGATCCCAGACGCTCCGCG
ATACTGCTGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCTATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11882.71 Da
• Isoelectric Point: 6.4751

>AT26982 WP_003159602.1 NC_017548:c4658966-4658643 [Pseudomonas aeruginosa M18]
MAKKFSDLRAQMSPEGQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLHVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT26982 NC_017548:c4658966-4658643 [Pseudomonas aeruginosa M18]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGGACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGGCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCACGTGC
AGCAGCCGGCAATTGCCAAAATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAACTGGAAGTGATTGCACGCTTCCCGGATGGCGCGGTGAAAATCAGCAACTTCGCGGATATCGAAGAGGCGGGCGC
CTAA

Structures

Toxin

• AlphaFold DB: V6AKP0

Antitoxin

• AlphaFold DB: A0A1C7BMJ2