Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 628325..629124 Replicon chromosome
Accession NZ_CP117013
Organism Escherichia coli strain MLI114

Toxin (Protein)


Gene name yhaV Uniprot ID A0A7B2BI69
Locus tag NL414_RS03475 Protein ID WP_000347258.1
Coordinates 628325..628789 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A368IY36
Locus tag NL414_RS03480 Protein ID WP_001604880.1
Coordinates 628789..629124 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL414_RS03445 (623326) 623326..623760 - 435 WP_000948829.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
NL414_RS03450 (623778) 623778..624656 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
NL414_RS03455 (624646) 624646..625425 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
NL414_RS03460 (625436) 625436..625909 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
NL414_RS03465 (625932) 625932..627212 - 1281 WP_000681943.1 tagatose-bisphosphate aldolase subunit KbaZ -
NL414_RS03470 (627461) 627461..628270 + 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
NL414_RS03475 (628325) 628325..628789 - 465 WP_000347258.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
NL414_RS03480 (628789) 628789..629124 - 336 WP_001604880.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
NL414_RS03485 (629273) 629273..630844 - 1572 WP_001273737.1 galactarate dehydratase -
NL414_RS03490 (631219) 631219..632553 + 1335 WP_000599654.1 galactarate/glucarate/glycerate transporter GarP -
NL414_RS03495 (632569) 632569..633339 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17844.32 Da        Isoelectric Point: 9.6924

>T269809 WP_000347258.1 NZ_CP117013:c628789-628325 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEILKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12340.91 Da        Isoelectric Point: 4.8616

>AT269809 WP_001604880.1 NZ_CP117013:c629124-628789 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGLDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7B2BI69

Antitoxin

Source ID Structure
AlphaFold DB A0A368IY36

References