Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3634834..3635528 Replicon chromosome
Accession NZ_CP117010
Organism Escherichia coli strain MLI110

Toxin (Protein)


Gene name yafO Uniprot ID S1EXB8
Locus tag NL413_RS18330 Protein ID WP_001263493.1
Coordinates 3634834..3635232 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag NL413_RS18335 Protein ID WP_000554757.1
Coordinates 3635235..3635528 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3630494) 3630494..3630574 - 81 NuclAT_11 - -
- (3630494) 3630494..3630574 - 81 NuclAT_11 - -
- (3630494) 3630494..3630574 - 81 NuclAT_11 - -
- (3630494) 3630494..3630574 - 81 NuclAT_11 - -
NL413_RS18300 (3629834) 3629834..3631078 - 1245 WP_000189541.1 esterase FrsA -
NL413_RS18305 (3631170) 3631170..3631628 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
NL413_RS18310 (3631889) 3631889..3633346 + 1458 WP_001293003.1 cytosol nonspecific dipeptidase -
NL413_RS18315 (3633403) 3633403..3633924 - 522 Protein_3450 peptide chain release factor H -
NL413_RS18320 (3633923) 3633923..3634126 - 204 Protein_3451 RtcB family protein -
NL413_RS18325 (3634372) 3634372..3634824 - 453 WP_001059847.1 GNAT family N-acetyltransferase -
NL413_RS18330 (3634834) 3634834..3635232 - 399 WP_001263493.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
NL413_RS18335 (3635235) 3635235..3635528 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
NL413_RS18340 (3635580) 3635580..3636635 - 1056 WP_001226164.1 DNA polymerase IV -
NL413_RS18345 (3636706) 3636706..3637491 - 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -
NL413_RS18350 (3637463) 3637463..3639175 + 1713 Protein_3457 flagellar biosynthesis protein FlhA -
NL413_RS18355 (3639399) 3639399..3639896 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15472.83 Da        Isoelectric Point: 8.0949

>T269802 WP_001263493.1 NZ_CP117010:c3635232-3634834 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT269802 WP_000554757.1 NZ_CP117010:c3635528-3635235 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1EXB8

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References