Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 5000224..5000826 Replicon chromosome
Accession NZ_CP117008
Organism Escherichia coli strain MLI109

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag NL412_RS25155 Protein ID WP_000897305.1
Coordinates 5000515..5000826 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NL412_RS25150 Protein ID WP_000356395.1
Coordinates 5000224..5000514 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL412_RS25115 (4995848) 4995848..4996750 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
NL412_RS25120 (4996747) 4996747..4997382 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
NL412_RS25125 (4997379) 4997379..4998308 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
NL412_RS25130 (4998490) 4998490..4998732 - 243 WP_001309881.1 CopG family transcriptional regulator -
NL412_RS25135 (4998951) 4998951..4999169 - 219 WP_001295676.1 CopG family transcriptional regulator -
NL412_RS25140 (4999588) 4999588..4999866 - 279 WP_001315112.1 hypothetical protein -
NL412_RS25145 (4999918) 4999918..5000139 - 222 WP_001550354.1 hypothetical protein -
NL412_RS25150 (5000224) 5000224..5000514 - 291 WP_000356395.1 NadS family protein Antitoxin
NL412_RS25155 (5000515) 5000515..5000826 - 312 WP_000897305.1 hypothetical protein Toxin
NL412_RS25160 (5001055) 5001055..5001963 + 909 WP_001550353.1 alpha/beta hydrolase -
NL412_RS25165 (5002131) 5002131..5003045 - 915 WP_109553727.1 transposase -
NL412_RS25170 (5003058) 5003058..5003945 - 888 Protein_4753 hypothetical protein -
NL412_RS25175 (5004361) 5004361..5005302 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
NL412_RS25180 (5005347) 5005347..5005784 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T269786 WP_000897305.1 NZ_CP117008:c5000826-5000515 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10869.46 Da        Isoelectric Point: 10.8907

>AT269786 WP_000356395.1 NZ_CP117008:c5000514-5000224 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References