Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1196300..1197027 Replicon chromosome
Accession NZ_CP117008
Organism Escherichia coli strain MLI109

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag NL412_RS06575 Protein ID WP_000547555.1
Coordinates 1196300..1196611 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NL412_RS06580 Protein ID WP_000126294.1
Coordinates 1196608..1197027 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL412_RS06550 (1192235) 1192235..1193944 + 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -
NL412_RS06555 (1193954) 1193954..1194496 + 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
NL412_RS06560 (1194496) 1194496..1195263 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
NL412_RS06565 (1195260) 1195260..1195670 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
NL412_RS06570 (1195663) 1195663..1196133 + 471 WP_001551542.1 hydrogenase maturation peptidase HycI -
NL412_RS06575 (1196300) 1196300..1196611 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
NL412_RS06580 (1196608) 1196608..1197027 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
NL412_RS06585 (1197106) 1197106..1198530 - 1425 WP_000110355.1 6-phospho-beta-glucosidase AscB -
NL412_RS06590 (1198539) 1198539..1199996 - 1458 WP_001107889.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
NL412_RS06595 (1200256) 1200256..1201266 + 1011 WP_029130985.1 DNA-binding transcriptional regulator AscG -
NL412_RS06600 (1201415) 1201415..1201942 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T269769 WP_000547555.1 NZ_CP117008:1196300-1196611 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT269769 WP_000126294.1 NZ_CP117008:1196608-1197027 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure

References