Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 628897..629696 Replicon chromosome
Accession NZ_CP117008
Organism Escherichia coli strain MLI109

Toxin (Protein)


Gene name yhaV Uniprot ID F0JQM9
Locus tag NL412_RS03830 Protein ID WP_000347270.1
Coordinates 628897..629361 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A0V9H1L4
Locus tag NL412_RS03835 Protein ID WP_001551693.1
Coordinates 629361..629696 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL412_RS03800 (623898) 623898..624332 - 435 WP_000948837.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
NL412_RS03805 (624350) 624350..625228 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
NL412_RS03810 (625218) 625218..625997 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
NL412_RS03815 (626008) 626008..626481 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
NL412_RS03820 (626504) 626504..627784 - 1281 WP_001551694.1 tagatose-bisphosphate aldolase subunit KbaZ -
NL412_RS03825 (628033) 628033..628842 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
NL412_RS03830 (628897) 628897..629361 - 465 WP_000347270.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
NL412_RS03835 (629361) 629361..629696 - 336 WP_001551693.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
NL412_RS03840 (629845) 629845..631416 - 1572 WP_001273738.1 galactarate dehydratase -
NL412_RS03845 (631791) 631791..633125 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
NL412_RS03850 (633141) 633141..633911 + 771 WP_001551692.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T269765 WP_000347270.1 NZ_CP117008:c629361-628897 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12347.87 Da        Isoelectric Point: 4.6890

>AT269765 WP_001551693.1 NZ_CP117008:c629696-629361 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NQ90

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9H1L4

References