Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1094441..1095168 Replicon chromosome
Accession NZ_CP117006
Organism Escherichia coli strain MLI108K3

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag NL411_RS06880 Protein ID WP_000547555.1
Coordinates 1094441..1094752 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NL411_RS06885 Protein ID WP_000126294.1
Coordinates 1094749..1095168 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL411_RS06855 (1090376) 1090376..1092085 + 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -
NL411_RS06860 (1092095) 1092095..1092637 + 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
NL411_RS06865 (1092637) 1092637..1093404 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
NL411_RS06870 (1093401) 1093401..1093811 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
NL411_RS06875 (1093804) 1093804..1094274 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
NL411_RS06880 (1094441) 1094441..1094752 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
NL411_RS06885 (1094749) 1094749..1095168 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
NL411_RS06890 (1095247) 1095247..1096671 - 1425 WP_000110355.1 6-phospho-beta-glucosidase AscB -
NL411_RS06895 (1096680) 1096680..1098137 - 1458 WP_001107889.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
NL411_RS06900 (1098397) 1098397..1099407 + 1011 WP_029130985.1 DNA-binding transcriptional regulator AscG -
NL411_RS06905 (1099556) 1099556..1100083 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T269740 WP_000547555.1 NZ_CP117006:1094441-1094752 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT269740 WP_000126294.1 NZ_CP117006:1094749-1095168 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure

References