Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 6520..7232 Replicon plasmid p92_MLI108-1
Accession NZ_CP116989
Organism Escherichia coli strain MLI108K1

Toxin (Protein)


Gene name higB Uniprot ID I2UJZ0
Locus tag NL409_RS01085 Protein ID WP_000162415.1
Coordinates 6520..6822 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NL409_RS01090 Protein ID WP_032220986.1
Coordinates 6894..7232 (+) Length 113 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL409_RS01055 (NL409_001055) 1539..2159 - 621 WP_044862276.1 hypothetical protein -
NL409_RS01060 (NL409_001060) 2203..3108 - 906 WP_080028513.1 recombination-associated protein RdgC -
NL409_RS01065 (NL409_001065) 3168..3821 - 654 WP_197347754.1 baseplate protein -
NL409_RS01070 (NL409_001070) 3818..4798 - 981 WP_000046500.1 hypothetical protein -
NL409_RS01075 (NL409_001075) 4802..5569 - 768 WP_197347753.1 baseplate -
NL409_RS01080 (NL409_001080) 5566..6357 - 792 WP_197347752.1 phage tail protein -
NL409_RS01085 (NL409_001085) 6520..6822 + 303 WP_000162415.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NL409_RS01090 (NL409_001090) 6894..7232 + 339 WP_032220986.1 HigA family addiction module antitoxin Antitoxin
NL409_RS01095 (NL409_001095) 7289..7474 + 186 Protein_10 hypothetical protein -
NL409_RS01100 (NL409_001100) 7511..8239 + 729 WP_197347751.1 hypothetical protein -
NL409_RS01105 (NL409_001105) 8273..9466 - 1194 WP_097433648.1 phage tail protein -
NL409_RS01110 (NL409_001110) 9459..9788 - 330 WP_000542383.1 hypothetical protein -
NL409_RS01115 (NL409_001115) 10117..10770 - 654 WP_001557715.1 hypothetical protein -
NL409_RS01120 (NL409_001120) 11039..11662 + 624 WP_202117614.1 hypothetical protein -
NL409_RS01125 (NL409_001125) 11683..12075 + 393 WP_000824759.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..92487 92487


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(6-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-69)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11818.56 Da        Isoelectric Point: 9.8739

>T269675 WP_000162415.1 NZ_CP116989:6520-6822 [Escherichia coli]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Download         Length: 303 bp

Antitoxin


Download         Length: 113 a.a.        Molecular weight: 13068.01 Da        Isoelectric Point: 6.4754

>AT269675 WP_032220986.1 NZ_CP116989:6894-7232 [Escherichia coli]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNMNKKAA

Download         Length: 339 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A631VBQ3

Antitoxin

Source ID Structure

References