Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 608902..609701 Replicon chromosome
Accession NZ_CP116987
Organism Escherichia coli strain MLI107

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag NL408_RS02975 Protein ID WP_000347267.1
Coordinates 608902..609366 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag NL408_RS02980 Protein ID WP_001307405.1
Coordinates 609366..609701 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL408_RS02945 (603903) 603903..604337 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
NL408_RS02950 (604355) 604355..605233 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
NL408_RS02955 (605223) 605223..606002 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
NL408_RS02960 (606013) 606013..606486 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
NL408_RS02965 (606509) 606509..607789 - 1281 WP_000681936.1 tagatose-bisphosphate aldolase subunit KbaZ -
NL408_RS02970 (608038) 608038..608847 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
NL408_RS02975 (608902) 608902..609366 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
NL408_RS02980 (609366) 609366..609701 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
NL408_RS02985 (609850) 609850..611421 - 1572 WP_001273741.1 galactarate dehydratase -
NL408_RS02990 (611796) 611796..613130 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
NL408_RS02995 (613146) 613146..613916 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 608902..620470 11568


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T269648 WP_000347267.1 NZ_CP116987:c609366-608902 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT269648 WP_001307405.1 NZ_CP116987:c609701-609366 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References