Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3911192..3911991 Replicon chromosome
Accession NZ_CP116981
Organism Escherichia coli strain E6474

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag PO910_RS18925 Protein ID WP_000347266.1
Coordinates 3911527..3911991 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag PO910_RS18920 Protein ID WP_001307405.1
Coordinates 3911192..3911527 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PO910_RS18905 (3906978) 3906978..3907748 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
PO910_RS18910 (3907764) 3907764..3909098 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
PO910_RS18915 (3909473) 3909473..3911043 + 1571 Protein_3690 galactarate dehydratase -
PO910_RS18920 (3911192) 3911192..3911527 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
PO910_RS18925 (3911527) 3911527..3911991 + 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
PO910_RS18930 (3912046) 3912046..3912855 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
PO910_RS18935 (3913104) 3913104..3914384 + 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
PO910_RS18940 (3914407) 3914407..3914880 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
PO910_RS18945 (3914891) 3914891..3915670 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
PO910_RS18950 (3915660) 3915660..3916538 + 879 WP_001315856.1 PTS N-acetylgalactosamine transporter subunit IID -
PO910_RS18955 (3916556) 3916556..3916990 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3900537..3911991 11454


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T269635 WP_000347266.1 NZ_CP116981:3911527-3911991 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT269635 WP_001307405.1 NZ_CP116981:3911192-3911527 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References