Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3979875..3980569 Replicon chromosome
Accession NZ_CP116976
Organism Escherichia coli strain CUVM53

Toxin (Protein)


Gene name yafO Uniprot ID S1EXB8
Locus tag PQP10_RS19270 Protein ID WP_001263493.1
Coordinates 3980171..3980569 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag PQP10_RS19265 Protein ID WP_000554757.1
Coordinates 3979875..3980168 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PQP10_RS19245 (3975515) 3975515..3976012 + 498 WP_000006261.1 REP-associated tyrosine transposase RayT -
PQP10_RS19250 (3976228) 3976228..3977940 - 1713 Protein_3749 flagellar biosynthesis protein FlhA -
PQP10_RS19255 (3977912) 3977912..3978697 + 786 WP_072132640.1 putative lateral flagellar export/assembly protein LafU -
PQP10_RS19260 (3978768) 3978768..3979823 + 1056 WP_001226164.1 DNA polymerase IV -
PQP10_RS19265 (3979875) 3979875..3980168 + 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
PQP10_RS19270 (3980171) 3980171..3980569 + 399 WP_001263493.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
PQP10_RS19275 (3980579) 3980579..3981031 + 453 WP_001059847.1 GNAT family N-acetyltransferase -
PQP10_RS19280 (3981277) 3981277..3981480 + 204 Protein_3755 RtcB family protein -
PQP10_RS19285 (3981479) 3981479..3982000 + 522 Protein_3756 peptide chain release factor H -
PQP10_RS19290 (3982057) 3982057..3983514 - 1458 WP_001293003.1 cytosol nonspecific dipeptidase -
PQP10_RS19295 (3983775) 3983775..3984233 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (3984829) 3984829..3984909 + 81 NuclAT_10 - -
- (3984829) 3984829..3984909 + 81 NuclAT_10 - -
- (3984829) 3984829..3984909 + 81 NuclAT_10 - -
- (3984829) 3984829..3984909 + 81 NuclAT_10 - -
PQP10_RS19300 (3984325) 3984325..3985569 + 1245 WP_000189541.1 esterase FrsA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15472.83 Da        Isoelectric Point: 8.0949

>T269602 WP_001263493.1 NZ_CP116976:3980171-3980569 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT269602 WP_000554757.1 NZ_CP116976:3979875-3980168 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1EXB8

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References