Detailed information of TA system TA026958

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 3272345..3272991
• Replicon: chromosome
• Accession: NC_017538
• Organism: Oligotropha carboxidovorans OM4

Toxin (Protein)

• Gene name: graT
• Uniprot ID: B6JDM4
• Locus tag: OCA4_RS15495
• Protein ID: WP_012561984.1
• Coordinates: 3272345..3272626 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: OCA4_RS15500
• Protein ID: WP_012561983.1
• Coordinates: 3272635..3272991 (+)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCA4_RS15455	3267362..3267946	-	585	WP_013913374.1	hypothetical protein	-
OCA4_RS15460	3268135..3268353	+	219	WP_012561990.1	hypothetical protein	-
OCA4_RS15465	3268458..3269177	+	720	WP_013913375.1	DUF3489 domain-containing protein	-
OCA4_RS15470	3269303..3269533	+	231	WP_012561988.1	hexameric tyrosine-coordinated heme protein	-
OCA4_RS18710	3269551..3270627	-	1077	WP_012561987.1	DNA cytosine methyltransferase	-
OCA4_RS15490	3270629..3271996	-	1368	WP_012561986.1	site-specific DNA-methyltransferase	-
OCA4_RS15495	3272345..3272626	+	282	WP_012561984.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OCA4_RS15500	3272635..3272991	+	357	WP_012561983.1	HigA family addiction module antidote protein	Antitoxin
OCA4_RS15505	3273389..3273940	-	552	WP_012561981.1	hypothetical protein	-
OCA4_RS15510	3273937..3274152	-	216	WP_012561980.1	hypothetical protein	-
OCA4_RS15515	3274149..3274466	-	318	WP_013913377.1	hypothetical protein	-
OCA4_RS15520	3274463..3274999	-	537	WP_012561978.1	hypothetical protein	-
OCA4_RS15525	3275332..3275580	-	249	WP_012561977.1	hypothetical protein	-
OCA4_RS15530	3275616..3275879	-	264	WP_013913378.1	SHOCT domain-containing protein	-
OCA4_RS15535	3275900..3276283	-	384	WP_012561975.1	DUF302 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3234586..3305891	71305

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (25-111)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10592.14 Da
• Isoelectric Point: 10.1526

>T26958 WP_012561984.1 NC_017538:3272345-3272626 [Afipia carboxidovorans OM4]
VIKSFGDKRTAAIFAGYAVRGLPPPIQERARAKLLVIDAAKQLDDLRQPPGNRLEALAGHRKGQHSIRINDQWRICFVWR
NGETFDVEIVDYH

Nucleotide

• Length: 282 bp

>T26958 NC_017538:3272345-3272626 [Afipia carboxidovorans OM4]
GTGATCAAGAGCTTCGGCGATAAACGCACCGCCGCAATCTTCGCAGGCTACGCCGTGCGCGGTTTGCCGCCGCCGATTCA
AGAGCGGGCTCGGGCGAAGCTGTTGGTGATCGACGCAGCAAAGCAACTCGACGATCTGCGCCAGCCGCCAGGCAACAGAC
TGGAGGCGTTGGCGGGTCACCGTAAGGGCCAGCACAGTATCCGCATCAACGATCAATGGCGGATATGCTTTGTGTGGCGG
AATGGCGAAACCTTCGATGTCGAAATTGTCGACTATCATTGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 13565.63 Da
• Isoelectric Point: 10.5936

>AT26958 WP_012561983.1 NC_017538:3272635-3272991 [Afipia carboxidovorans OM4]
MMSIKRENIDKGEIDFADVRSGRRLPPIHPGTILRDEFLTPMQISVYELASAIKTPRSRINDIVLGRRTITTDTALRLGR
YLGTSPEFWINLQARYDLDVANRTVRRKIEQEITPRAA

Nucleotide

• Length: 357 bp

>AT26958 NC_017538:3272635-3272991 [Afipia carboxidovorans OM4]
ATGATGAGCATCAAGCGTGAAAACATCGACAAGGGAGAGATCGACTTCGCCGACGTGCGTTCCGGGCGACGGCTGCCGCC
AATCCATCCGGGCACGATCCTCCGCGATGAGTTCCTGACCCCTATGCAGATCAGCGTCTATGAGCTTGCCAGCGCAATCA
AGACGCCACGTTCGCGGATCAACGACATCGTACTGGGGCGGCGCACGATTACCACCGATACCGCACTACGGCTCGGACGC
TATCTTGGAACCTCGCCGGAGTTCTGGATCAATCTGCAGGCGCGCTACGATCTCGATGTCGCCAATCGCACCGTTCGCCG
TAAGATCGAACAGGAGATCACGCCACGTGCAGCTTGA

Structures

Toxin

• AlphaFold DB: B6JDM4