Detailed information of TA system TA269575

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 135419..135844
• Replicon: plasmid pCUVM3.1
• Accession: NZ_CP116971
• Organism: Escherichia coli strain CUVM3

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: PQP09_RS24800
• Protein ID: WP_223195199.1
• Coordinates: 135419..135541 (-)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	135633..135844 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PQP09_RS24765 (130581)	130581..131402	-	822	WP_061856956.1	DUF932 domain-containing protein	-
PQP09_RS24770 (131521)	131521..131808	-	288	WP_000107543.1	hypothetical protein	-
PQP09_RS24775 (131833)	131833..132039	-	207	WP_024189527.1	hypothetical protein	-
PQP09_RS24780 (132109)	132109..132406	+	298	Protein_144	hypothetical protein	-
PQP09_RS24785 (132504)	132504..133154	+	651	WP_000993956.1	IS66-like element accessory protein TnpA	-
PQP09_RS24790 (133154)	133154..133501	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
PQP09_RS24795 (133521)	133521..135092	+	1572	WP_094083869.1	IS66-like element ISCro1 family transposase	-
PQP09_RS24800 (135419)	135419..135541	-	123	WP_223195199.1	Hok/Gef family protein	Toxin
PQP09_RS24805 (135486)	135486..135599	-	114	Protein_149	DUF5431 family protein	-
- (135633)	135633..135844	-	212	NuclAT_0	-	Antitoxin
- (135633)	135633..135844	-	212	NuclAT_0	-	Antitoxin
- (135633)	135633..135844	-	212	NuclAT_0	-	Antitoxin
- (135633)	135633..135844	-	212	NuclAT_0	-	Antitoxin
PQP09_RS24810 (135789)	135789..136575	-	787	Protein_150	plasmid SOS inhibition protein A	-
PQP09_RS24815 (136572)	136572..137006	-	435	WP_000845926.1	conjugation system SOS inhibitor PsiB	-
PQP09_RS24820 (137061)	137061..139020	-	1960	Protein_152	ParB/RepB/Spo0J family partition protein	-
PQP09_RS24825 (139086)	139086..139319	-	234	WP_000006002.1	DUF905 family protein	-
PQP09_RS24830 (139377)	139377..139805	-	429	WP_063115590.1	single-stranded DNA-binding protein	-
PQP09_RS24835 (140505)	140505..140696	-	192	WP_025269849.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..164599	164599

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-35)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4536.35 Da
• Isoelectric Point: 8.2691

>T269575 WP_223195199.1 NZ_CP116971:c135541-135419 [Escherichia coli]
IVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 123 bp

Antitoxin

Nucleotide

• Length: 212 bp

>AT269575 NZ_CP116971:c135844-135633 [Escherichia coli]
TCACACGGATTACCCGTGAACTGGTTGAACGACCAGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
AGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGC
AGAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Antitoxin