Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4143616..4144157 Replicon chromosome
Accession NZ_CP116970
Organism Escherichia coli strain CUVM3

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag PQP09_RS20445 Protein ID WP_000615976.1
Coordinates 4143616..4143894 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag PQP09_RS20450 Protein ID WP_000729704.1
Coordinates 4143897..4144157 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PQP09_RS20430 (4141198) 4141198..4141776 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
PQP09_RS20435 (4141982) 4141982..4142749 + 768 WP_000333380.1 class II glutamine amidotransferase -
PQP09_RS20440 (4142720) 4142720..4143460 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
PQP09_RS20445 (4143616) 4143616..4143894 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
PQP09_RS20450 (4143897) 4143897..4144157 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
PQP09_RS20455 (4144343) 4144343..4145116 + 774 WP_000543895.1 C40 family peptidase -
PQP09_RS20460 (4145173) 4145173..4145529 - 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
PQP09_RS20465 (4145522) 4145522..4145800 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
PQP09_RS20470 (4145905) 4145905..4147617 - 1713 Protein_3984 flagellar biosynthesis protein FlhA -
PQP09_RS20475 (4147589) 4147589..4148374 + 786 WP_000207590.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - rhs/PAAR / gmhA/lpcA 4127782..4145800 18018


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T269569 WP_000615976.1 NZ_CP116970:c4143894-4143616 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT269569 WP_000729704.1 NZ_CP116970:c4144157-4143897 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References