Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2469602..2470401 Replicon chromosome
Accession NZ_CP116970
Organism Escherichia coli strain CUVM3

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag PQP09_RS12330 Protein ID WP_000347267.1
Coordinates 2469937..2470401 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag PQP09_RS12325 Protein ID WP_001307405.1
Coordinates 2469602..2469937 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PQP09_RS12310 (2465387) 2465387..2466157 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
PQP09_RS12315 (2466173) 2466173..2467507 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
PQP09_RS12320 (2467882) 2467882..2469453 + 1572 WP_001273741.1 galactarate dehydratase -
PQP09_RS12325 (2469602) 2469602..2469937 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
PQP09_RS12330 (2469937) 2469937..2470401 + 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
PQP09_RS12335 (2470456) 2470456..2471265 - 810 WP_000072180.1 aga operon transcriptional regulator AgaR -
PQP09_RS12340 (2471514) 2471514..2472794 + 1281 WP_000681947.1 tagatose-bisphosphate aldolase subunit KbaZ -
PQP09_RS12345 (2472817) 2472817..2473290 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
PQP09_RS12350 (2473301) 2473301..2474080 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
PQP09_RS12355 (2474070) 2474070..2474948 + 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
PQP09_RS12360 (2474966) 2474966..2475400 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2457335..2470401 13066


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T269563 WP_000347267.1 NZ_CP116970:2469937-2470401 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT269563 WP_001307405.1 NZ_CP116970:2469602-2469937 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References