Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 69835..70427 Replicon plasmid p2939_90_1
Accession NZ_CP116944
Organism Providencia alcalifaciens strain 2939/90

Toxin (Protein)


Gene name higB Uniprot ID A0A6L6F789
Locus tag PO864_RS19770 Protein ID WP_036954335.1
Coordinates 70149..70427 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A6L6F9S8
Locus tag PO864_RS19765 Protein ID WP_036961084.1
Coordinates 69835..70149 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PO864_RS19755 (PO864_19960) 67498..68874 - 1377 WP_036974601.1 conjugal transfer pilus assembly protein TraH -
PO864_RS19760 (PO864_19965) 69286..69637 + 352 Protein_51 transposase -
PO864_RS19765 (PO864_19970) 69835..70149 - 315 WP_036961084.1 HigA family addiction module antitoxin Antitoxin
PO864_RS19770 (PO864_19975) 70149..70427 - 279 WP_036954335.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PO864_RS19775 (PO864_19980) 70460..71041 - 582 WP_282490695.1 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB -
PO864_RS19780 (PO864_19985) 71020..71340 - 321 WP_282490697.1 hypothetical protein -
PO864_RS19785 (PO864_19990) 71351..72142 - 792 Protein_56 type-F conjugative transfer system pilin assembly protein TraF -
PO864_RS19790 (PO864_20000) 72174..72821 - 648 WP_282490698.1 conjugal transfer protein TraN -
PO864_RS19795 (PO864_20005) 72718..73809 - 1092 WP_282490699.1 hypothetical protein -
PO864_RS19800 (PO864_20010) 73770..74522 - 753 WP_282490701.1 type-F conjugative transfer system pilin assembly protein TrbC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - sicA / spaS / spaQ / spaP / invC/sctN / invA / espC / prgK / prgI 1..127696 127696


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(10-67)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10857.23 Da        Isoelectric Point: 8.4391

>T269519 WP_036954335.1 NZ_CP116944:c70427-70149 [Providencia alcalifaciens]
MIKSFKHKGLKQLFEKGNTSGVPAQDAERINDRLQAIDTANDIRELDRQIYKLHPLKGDREGYWSITVRANWRITFQFIN
GDAYILNYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11431.05 Da        Isoelectric Point: 7.2028

>AT269519 WP_036961084.1 NZ_CP116944:c70149-69835 [Providencia alcalifaciens]
MRQFKISHPGEIIARDLADMGISGRRFALNIGVTPATVSRFLSGKTALTPSLAIRIAAALGSTPEFWLRLQSAYDLRQLE
NKIDTSDIILYEEPSDSSEGISKH

Download         Length: 315 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6L6F789

Antitoxin

Source ID Structure
AlphaFold DB A0A6L6F9S8

References